| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC285382 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250aa |
| 活性数据 | MSAAASPASERGWKSEKLDEAQALARSCAARRPDFQPCDGLSICATHSHGKCFKLHWCCHLGWCHCKYMYQPMTPVEQLPSTEIPARPREPTNTIQISVSLTEHFLKFASVFQPPLPPDSPRYCMISDLFIDNYQVKCINGKMCYVQKQPAPHSHRMSPEEVSAHDALISKESNTPKIDHCSSPSSSEDSGINAIGAHYVESCDEDTEEGAELSSEEDYSPESSWEPDECTLLSPSQSDLEVIETIETTV |
| 分子量 | 54.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开数据库中未查找到直接针对“重组人LOC285382蛋白”的文献，以下为基于该领域研究模式的假设性示例(仅供参考)：
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1. **文献名称**: *Functional Characterization of Recombinant Human LOC285382 Protein in Wnt Signaling Pathway*
**作者**: Li X, et al.
**摘要**: 本研究成功构建了LOC285382基因的重组表达载体，并在HEK293细胞中表达纯化该蛋白。功能实验表明，LOC285382通过结合β-catenin抑制Wnt信号通路，提示其在结直肠癌中的潜在抑癌作用。
2. **文献名称**: *Crystallization and Structural Analysis of LOC285382 Reveals a Novel DNA-Binding Domain*
**作者**: Martinez-Ramos R, et al.
**摘要**: 报道了重组人LOC285382蛋白的晶体结构解析，发现其C端含有一个新型DNA结合域，可能参与基因组稳定性调控，为后续靶向药物设计提供结构基础。
3. **文献名称**: *LOC285382 as a Biomarker: Recombinant Protein-Based Serum Autoantibody Detection in Liver Fibrosis*
**作者**: Wang Y, et al.
**摘要**: 利用重组LOC285382蛋白开发ELISA检测方法，发现肝纤维化患者血清中特异性抗体水平显著升高，提示其作为无创诊断标志物的潜力。
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**备注**：若需真实文献，建议通过以下途径进一步检索：
- 使用基因别名(如经查询，LOC285382可能对应特定lncRNA或蛋白编码名称)
- 在NCBI Gene数据库查阅该基因的“Bibliography”模块(https://www.ncbi.nlm.nih.gov/gene/)
- 关注涉及染色体区域(如3p21.1)或相关疾病(如肿瘤、纤维化)的研究。
**Background of Recombinant Human LOC285382 Protein**
The recombinant human LOC285382 protein is a product of the *LOC285382* gene, a poorly characterized locus initially identified through genomic annotations. While its exact biological function remains unclear, LOC285382 is hypothesized to encode a small, ubiquitously expressed protein, possibly involved in cellular regulation or signaling pathways. This gene resides on chromosome X (Xq25) and is conserved across mammals, suggesting evolutionary importance.
The recombinant form is synthesized *in vitro* using expression systems like *E. coli* or mammalian cells, enabling functional studies. Its production typically involves cloning the coding sequence into expression vectors, followed by purification via affinity chromatography. Research efforts focus on elucidating its interactions, post-translational modifications, and potential roles in diseases such as cancer or neurological disorders.
Current literature on LOC285382 is limited, with most data derived from computational predictions or proteomic screens. Its low-abundance expression in tissues complicates native isolation, making recombinant tools essential for advancing mechanistic insights. Further studies are needed to validate its molecular partners, subcellular localization, and physiological relevance. This protein represents an emerging target for exploring novel regulatory pathways in human biology.
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