| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC221091 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 活性数据 | MLGSPCLLWLLAVTFLVPRAQPLAPQDFEEEEADETETAWPPLPAVPCDYDHCRHLQVPCKELQRVGPAACLCPGLSSPAQPPDPPRMGEVRIAAEEGRAVVHWCAPFSPVLHYWLLLWDGSEAAQKGPPLNATVRRAELKGLKPGGIYVVCVVAANEAGASRVPQAGGEGLEGADIPAFGPCSRLAVPPNPRTLVHAAVGVGTALALLSCAALVWHFCLRDRWGCPRRAAARAAGAL |
| 分子量 | 51.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOC221091蛋白的3篇假设性参考文献示例(注：LOC221091为虚构编号，实际研究中需核实基因/蛋白名称，以下内容仅供示例参考)：
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1. **文献名称**：Expression and Functional Analysis of Recombinant Human LOC221091 Protein in Bacterial Systems
**作者**：Zhang L. et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人LOC221091蛋白，并通过Ni柱亲和层析纯化获得高纯度产物。实验发现该蛋白在体外具有ATP结合活性，提示其可能参与细胞能量代谢相关通路。
2. **文献名称**：Structural Characterization of LOC221091 and Its Interaction with TGF-β Signaling Pathway
**作者**：Kim S., Patel R.
**摘要**：通过X射线晶体学解析了重组人LOC221091的三维结构，发现其含有保守的SH2结构域。进一步实验表明，该蛋白通过结合TGF-β受体调控SMAD蛋白磷酸化，可能影响肿瘤细胞转移。
3. **文献名称**：LOC221091 Knockdown Alters Inflammatory Response in Macrophages: Implications for Autoimmune Diseases
**作者**：García-Molina V. et al.
**摘要**：利用重组LOC221091蛋白及RNA干扰技术，发现该蛋白通过抑制NF-κB通路减少巨噬细胞中IL-6和TNF-α的释放，提示其在类风湿性关节炎等疾病中的潜在治疗价值。
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**说明**：
- 上述文献为示例性质，实际研究中LOC221091可能对应不同基因/蛋白，建议通过NCBI Gene/UniProt核实编号准确性，并使用PubMed/Web of Science等数据库检索真实文献。
- 重组蛋白研究通常涵盖表达系统优化(如哺乳动物/昆虫细胞)、功能验证(酶活性/信号通路互作)及疾病模型应用等方向，可结合具体研究目标筛选文献。
Recombinant human LOC221091 protein is a genetically engineered form of a poorly characterized protein encoded by the LOC221091 gene, currently listed as a provisional identifier in genomic databases. This gene is mapped to chromosome 19 (19q13.12) and encodes a putative protein with unclear physiological roles. Bioinformatics analyses suggest it may belong to a class of secreted or membrane-associated proteins due to predicted signal peptides and transmembrane domains, though experimental validation remains limited.
The recombinant version is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian or insect cell) expression systems, enabling studies on its structure and function. Its recombinant production allows for applications in antibody development, protein interaction assays, and cellular pathway analyses. Preliminary studies hint at potential involvement in cell adhesion, signaling, or immune modulation, but mechanistic insights are scarce.
Interest in LOC221091 has emerged from high-throughput omics studies linking its expression patterns to cancers, neurological disorders, and metabolic diseases. For instance, elevated mRNA levels have been noted in tumor tissues, suggesting a possible role in oncogenesis or tumor microenvironment regulation. However, these associations remain correlative, and functional studies using recombinant protein are needed to establish causality.
Overall, LOC221091 represents an understudied protein with potential biomedical relevance. Its recombinant form serves as a critical tool for unraveling its biological significance and therapeutic/diagnostic potential, though substantial research gaps persist.
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