| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC197350 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183aa |
| 活性数据 | MAHGGPRGQLLGADGQEVQPEALMQELSRCQVLQGRPKIFLLQACRGGNRDAGVGPTALPWYWSWLRAPPSVPSHADVLQIYAEAQGSSCRGTPPGSSDQADILTVYSAAEGYVAYRDDKGSDFIQTLVEVLRANPGRDLLELLTEVNRRVCEQEVLGPDCDELRKACLEIRSSLRRRLCLQA |
| 分子量 | 46.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOC197350(CCDC185)蛋白的假设参考文献示例(基于可能的关联研究方向)：
1. **文献名称**：**"Expression and Functional Analysis of Recombinant CCDC185 in Human Cell Lines"**
**作者**：Chen, L. et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组人CCDC185蛋白，并发现其定位于线粒体膜间隙。功能实验表明，CCDC185敲低导致线粒体膜电位下降，提示其在能量代谢中可能发挥调控作用。
2. **文献名称**：**"Structural Insights into CCDC185: Purification and Crystallization of the Recombinant Protein"**
**作者**：Wang, Y. & Kim, S.
**摘要**：作者利用昆虫细胞表达系统重组表达CCDC185.通过亲和层析纯化获得高纯度蛋白，并成功解析其晶体结构。结构分析显示其具有典型的卷曲螺旋域，为研究蛋白互作机制奠定基础。
3. **文献名称**：**"CCDC185 Interaction with Respiratory Complex I: A Study Using Recombinant Protein Probes"**
**作者**：Gupta, R. et al.
**摘要**：通过重组CCDC185蛋白的GST pull-down实验，鉴定了其与线粒体复合物I的亚基NDUFS2直接互作，提示其可能在氧化磷酸化调控中具有功能。
4. **文献名称**：**"Development of a Polyclonal Antibody Against Recombinant Human CCDC185 for Diagnostic Applications"**
**作者**：Martinez, P. et al.
**摘要**：研究利用大肠杆菌表达的重组CCDC185免疫家兔制备多克隆抗体，验证了抗体在Western blot和免疫组化中的特异性，为疾病标志物研究提供工具。
**注意**：以上文献为模拟示例，实际研究中LOC197350可能已更名或研究较少，建议通过基因别名(如CCDC185)在PubMed、Google Scholar等平台检索最新文献。
Recombinant human LOC197350 protein is a biologically engineered form of the protein encoded by the LOC197350 gene, a poorly characterized gene in current scientific literature. Located on chromosome 3 in humans, the gene's exact biological function remains unclear, though computational analyses suggest it may encode a secreted or membrane-associated protein due to predicted signal peptides and transmembrane domains. Structural modeling indicates potential globular folds with conserved motifs, hinting at possible roles in cellular signaling or protein-protein interactions.
Recombinant production of LOC197350 typically involves cloning its coding sequence into expression vectors (e.g., E. coli or mammalian systems) to enable large-scale purification. This engineered protein serves as a critical tool for functional studies, allowing researchers to investigate its biochemical properties, interaction partners, and potential involvement in disease pathways. Current research focuses on characterizing its expression patterns across tissues and exploring associations with specific cancers or metabolic disorders through proteomic screenings. Challenges include optimizing expression conditions due to putative post-translational modifications and solubility issues. Antibodies against recombinant LOC197350 are being developed for immunohistochemistry and ELISA-based detection in clinical samples. While its physiological significance remains elusive, the recombinant protein provides a foundation for unraveling its molecular mechanisms and therapeutic potential. Further studies may clarify its role as a biomarker or drug target in precision medicine applications.
×
