| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LNX2 |
| Uniprot No | Q8N448 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690aa |
| 活性数据 | MGTTSDEMVSVEQTSSSSLNPLCFECGQQHWTRENHLYNYQNEVDDDLVCHICLQPLLQPLDTPRGHTFCYKCLRNFLQEKDFCPLDRKRLHFKLCKKSSILVHKLLDKLLVLCPFSSVCKDVMQRCDLEAHLKNRCPGASHRRVALERRKTSRTQAEIENENGPTLLDPAGTLSPEADCLGTGAVPVERHLTSASLPTWSEEPGLDNPAFEESAGADTTQQPLSLPEGEITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNISNVSHNYARAVLSQPCNTLHLTVLRERRFGNRAHNHSDSNSPREEIFQVALHKRDSGEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTPPPYYSRPSSHKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLKASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHSCHDIVLRRSYLGSWGFSIVGGYEENHTNQPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV |
| 分子量 | 102.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LNX2蛋白的3篇代表性文献示例(虚构内容，仅供参考)：
1. **标题**：*"Recombinant Human LNX2 Expression and Its E3 Ubiquitin Ligase Activity Analysis"*
**作者**：Zhang, Y. et al. (2018)
**摘要**：研究报道了人源LNX2的重组表达及纯化方案，利用大肠杆菌系统成功获得可溶性蛋白，并证实其通过RING结构域介导的泛素化活性，为后续功能研究提供基础。
2. **标题**：*"LNX2 Interacts with Numb to Regulate Notch Signaling in Neural Stem Cells"*
**作者**：Chen, L. & Smith, J. (2020)
**摘要**：通过体外重组LNX2蛋白与Numb蛋白的结合实验，发现LNX2通过泛素化降解NUMB，增强Notch信号通路活性，从而影响神经干细胞的命运决定。
3. **标题**：*"Crystal Structure of the PDZ Domains in LNX2 Reveals Substrate Recognition Mechanism"*
**作者**：Kumar, R. et al. (2021)
**摘要**：解析了重组人LNX2蛋白中PDZ结构域的晶体结构，阐明其通过特异性识别C端肽段与多种跨膜蛋白(如ErbB2)结合，提示其在细胞膜蛋白调控中的潜在作用。
注：上述文献为示例性内容，实际研究中请通过权威数据库(如PubMed、Web of Science)查询真实文献。
Ligand of Numb Protein-X 2 (LNX2) is a member of the LNX family of E3 ubiquitin ligases, which play critical roles in regulating protein stability, localization, and signaling pathways through ubiquitination. Human LNX2 contains a characteristic RING domain, enabling its enzymatic activity in tagging target proteins with ubiquitin for proteasomal degradation or functional modulation. It is best known for interacting with NUMB, a key cell fate determinant, thereby influencing Notch signaling—a pathway essential for cell differentiation, development, and homeostasis. By ubiquitinating NUMB, LNX2 may regulate asymmetric cell division, neuronal development, and tissue patterning.
Recombinant human LNX2 protein, produced via expression systems like *E. coli* or mammalian cells, is widely used to study its biochemical properties, substrate interactions, and enzymatic mechanisms *in vitro*. Its role in diseases, particularly cancer, is under investigation, as dysregulated ubiquitination pathways are implicated in tumorigenesis and metastasis. Studies also explore LNX2's involvement in immune responses and neurological disorders, leveraging recombinant forms for structural analysis or functional assays. Despite progress, tissue-specific functions and regulatory networks of LNX2 remain areas of active research, highlighting its potential as a therapeutic target or diagnostic marker.
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