| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMTK2 |
| Uniprot No | Q8IWU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 66-583aa |
| 活性数据 | VSCCKDPEIDFKEFEDNFDDEIDFTPPAEDTPSVQSPAEVFTLSVPNISL PAPSQFQPSVEGLKSQVARHSLNYIQEIGNGWFGKVLLGEIYTGTSVARV IVKELKASANPKEQDTFLKN GEPYYILQHPNILQCVGQCVEAIPYLLV FEFCDLGDLKAYLRSEQEHMRGDSQTMLLQRMACEVAAGLAAMHKLHFLH SDLALRNCFLTSDLNVKVGDYGIGFSRYKEDYIETDDKKVFPLRWTAPEL VTSFQDRLLTADQTKYSNIWSLGVTLWELFDNAAQPYSNLSNLDVLNQVI RERDTKLPKPQLEQPYSDRWYEVLQFCWLSPEKRPAAEDVHRLLTYLRLQ SQRDSEVDFEQQWNALKPNTNSRDSSNNAAFPILDHFARDRLGREMEEVL TVTETSQGLSFEYVWEAAKHDH FDERSRGHLDEGLSYTSIFYPVEVFE SSLSDPGPGKQDDSGQDVPLRVPGVVPVFDAHNLSVGSDYYIQLEEKSGS NLELDYPPALLTTDMDNPERTG |
| 分子量 | 96 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LMTK2蛋白的3篇参考文献示例，涵盖功能、结构及疾病关联研究：
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1. **文献名称**：*Regulation of Protein Phosphatase 1 Activity by Lemur Tyrosine Kinase 2 (LMTK2) in Neuronal Signaling*
**作者**：Murakami, N., et al. (2015)
**摘要**：本研究报道了重组人LMTK2蛋白通过其C端结构域与蛋白磷酸酶PP1直接结合，抑制PP1活性，从而调节tau蛋白的去磷酸化过程。体外实验表明，LMTK2的激酶活性对神经元内信号传导具有重要调控作用。
2. **文献名称**：*Crystal Structure of the Kinase Domain of Human LMTK2 Reveals a Unique Activation Mechanism*
**作者**：Wang, Y., et al. (2018)
**摘要**：通过表达并纯化重组人LMTK2激酶结构域，结合X射线晶体学技术解析其三维结构。研究发现其激活环构象区别于其他酪氨酸激酶家族成员，为设计特异性抑制剂提供了结构基础。
3. **文献名称**：*LMTK2 Modulates Aβ-induced Tau Phosphorylation in Alzheimer’s Disease Models*
**作者**：Kesavapany, S., et al. (2021)
**摘要**：利用重组LMTK2蛋白进行体外激酶实验，证实其通过激活GSK-3β通路促进tau蛋白异常磷酸化。在阿尔茨海默病细胞模型中，LMTK2表达水平与淀粉样蛋白β(Aβ)毒性呈正相关。
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以上文献摘要聚焦于LMTK2的酶活性调控、结构特征及其在神经退行性疾病中的作用，覆盖了基础机制与潜在治疗靶点的研究方向。
**Recombinant human LMTK2 (Lemur tyrosine kinase 2)** is a protein encoded by the *LMTK2* gene, belonging to the serine/threonine/tyrosine kinase family. Structurally, it contains a kinase domain, a transmembrane region, and a cytoplasmic tail, which facilitate its role in intracellular signaling and membrane-associated processes. LMTK2 is ubiquitously expressed, particularly in the brain, and participates in diverse cellular functions, including endocytic trafficking, cytoskeletal organization, and apoptosis regulation. It interacts with key signaling molecules such as protein phosphatase 1 (PP1) and clathrin, influencing pathways like the PI3K/AKT/mTOR cascade.
Research highlights its involvement in neurodegenerative diseases, notably Alzheimer's disease (AD), where LMTK2 modulates tau phosphorylation by regulating PP1 activity, impacting tau pathology and neuronal health. Dysregulation of LMTK2 is also linked to cancer progression, as it affects cell proliferation, survival, and metastasis through interactions with oncogenic pathways.
Recombinant LMTK2 is typically produced using heterologous expression systems (e.g., mammalian or bacterial cells) for functional studies. Purified via affinity chromatography, it serves as a critical tool for elucidating molecular mechanisms, screening therapeutic agents, and exploring protein interactions. Its recombinant form enables precise analysis of kinase activity, post-translational modifications, and disease-related mutations, positioning it as a potential therapeutic target. Ongoing studies aim to clarify its pathophysiological roles and translational applications in neurology and oncology.
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