| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMOD1 |
| Uniprot No | P29536 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600aa |
| 活性数据 | MSRVAKYRRQ VSEDPDIDSL LETLSPEEME ELEKELDVVD PDGSVPVGLR QRNQTEKQST GVYNREAMLN FCEKETKKLM QREMSMDESK QVETKTDAKN GEERGRDASK KALGPRRDSD LGKEPKRGGL KKSFSRDRDE AGGKSGEKPK EEKIIRGIDK GRVRAAVDKK EAGKDGRGEE RAVATKKEEE KKGSDRNTGL SRDKDKKREE MKEVAKKEDD EKVKGERRNT DTRKEGEKMK RAGGNTDMKK EDEKVKRGTG NTDTKKDDEK VKKNEPLHEK EAKDDSKTKT PEKQTPSGPT KPSEGPAKVE EEAAPSIFDE PLERVKNNDP EMTEVNVNNS DCITNEILVR FTEALEFNTV VKLFALANTR ADDHVAFAIA IMLKANKTIT SLNLDSNHIT GKGILAIFRA LLQNNTLTEL RFHNQRHICG GKTEMEIAKL LKENTTLLKL GYHFELAGPR MTVTNLLSRN MDKQRQKRLQ EQRQAQEAKG EKKDLLEVPK AGAVAKGSPK PSPQPSPKPS PKNSPKKGGA PAAPPPPPPP LAPPLIMENL KNSLSPATQR KMGDKVLPAQ EKNSRDQLLA AIRSSNLKQL KKVEVPKLLQ |
| 分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LMOD1蛋白的3篇参考文献及相关摘要信息：
1. **文献名称**：*"Recombinant Human Leiomodin 1 Promotes Actin Nucleation and Polymerization In Vitro"*
**作者**：Smith J, et al.
**摘要**：该研究成功在大肠杆菌系统中表达并纯化了重组人LMOD1蛋白，证明其在体外显著促进肌动蛋白的成核和纤维延伸，表明LMOD1在调控细胞骨架动态中起关键作用。
2. **文献名称**：*"Functional Analysis of LMOD1 Mutations in Cardiomyopathy Using Recombinant Protein Models"*
**作者**：Zhang Y, et al.
**摘要**：研究者利用昆虫细胞表达体系制备重组LMOD1蛋白，分析致病突变对其功能的影响，发现突变体无法有效结合肌动蛋白，导致心肌细胞收缩功能缺陷，与遗传性心肌病相关。
3. **文献名称**：*"Structural Basis of Leiomodin 1-Mediated Actin Filament Assembly"*
**作者**：Chen R, et al.
**摘要**：通过重组人LMOD1蛋白的晶体结构解析，揭示了其羧基端结构域在肌动蛋白结合中的关键作用，为LMOD1调控细胞骨架组装的分子机制提供了结构生物学证据。
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*注：以上文献信息为示例，实际引用时建议通过PubMed或Web of Science等数据库核对文献详情。*
Leiomodin 1 (LMOD1) is an actin-binding protein predominantly expressed in smooth muscle and skeletal muscle cells. As a member of the leiomodin/whirlin protein family, it plays a critical role in regulating actin filament assembly and stabilization. LMOD1 functions as an actin nucleator, promoting the formation of elongated actin filaments by binding to tropomyosin and enhancing the polymerization of actin monomers. Its structural organization includes N-terminal leucine-rich repeats and a C-terminal actin-binding domain, enabling interactions with cytoskeletal components.
LMOD1 is essential for maintaining sarcomere integrity in muscle tissues. Studies link LMOD1 mutations to nemaline myopathy and congenital muscular dystrophy, highlighting its importance in muscle development and function. Recombinant human LMOD1 protein, produced via bacterial or eukaryotic expression systems, enables in vitro exploration of its biochemical properties, interaction partners, and disease mechanisms. This engineered protein serves as a tool for studying actin dynamics, myofibril organization, and potential therapeutic interventions. Challenges in recombinant production include preserving post-translational modifications and ensuring proper folding for functional studies. Current research focuses on clarifying its regulatory pathways and leveraging recombinant LMOD1 to model muscle disorders or screen drugs targeting actin-related pathologies.
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