| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMO2 |
| Uniprot No | P25791 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158aa |
| 活性数据 | MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRPFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGMI |
| 分子量 | 43.12 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LMO2蛋白的3篇参考文献示例(注：部分内容为示例性概括，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Structural insights into the interaction of LMO2 with the TAL1 transcription complex in leukemia"*
**作者**：Smith J, et al.
**摘要**：本研究解析了重组人LMO2蛋白与TAL1/E2A复合物的相互作用机制，通过X射线晶体学揭示了LMO2的LIM结构域如何介导转录复合物组装，为白血病中异常转录调控提供结构基础。
2. **文献名称**：*"Recombinant human LMO2 protein enhances hematopoietic stem cell expansion ex vivo"*
**作者**：Chen L, et al.
**摘要**：报道重组人LMO2蛋白在体外造血干细胞扩增中的应用，实验表明LMO2通过激活Notch信号通路促进干细胞的自我更新，为再生医学提供潜在工具。
3. **文献名称**：*"Functional characterization of LMO2 mutations in T-cell acute lymphoblastic leukemia using recombinant protein models"*
**作者**：Wang Y, et al.
**摘要**：通过表达携带白血病相关突变的重组LMO2蛋白，研究其与伴侣蛋白(如LDB1)的结合能力变化，发现特定突变破坏蛋白相互作用，导致异常基因表达和肿瘤发生。
如需具体文献，建议通过PubMed或Web of Science搜索关键词 **"recombinant human LMO2 protein"** 或 **"LMO2 structure/function"** 获取最新研究。
The LMO2 (LIM Domain Only 2) protein, a member of the LIM-only transcriptional regulatory family, plays pivotal roles in hematopoietic development and angiogenesis. Initially identified for its involvement in T-cell acute lymphoblastic leukemia (T-ALL) due to chromosomal translocations, LMO2 acts as a scaffolding adaptor protein, bridging transcription factors like TAL1. GATA, and LDB1 to modulate gene expression. Its activity is critical during embryogenesis, particularly in forming blood vessels and red/white blood cell lineages. Recombinant human LMO2 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms, structural interactions, and pathogenic contributions in hematologic cancers. Produced via bacterial (e.g., *E. coli*) or eukaryotic expression systems, the purified protein facilitates biochemical assays, crystallography, and disease modeling. Research leveraging recombinant LMO2 has highlighted its oncogenic potential when dysregulated, aiding therapeutic development targeting LMO2-associated pathways. Additionally, it serves as a tool to explore normal hematopoietic stem cell regulation and vascular development. Current investigations also focus on its role in solid tumors and neurological disorders, expanding its relevance beyond hematopoiesis.
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