| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMLN |
| Uniprot No | Q96KR4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-655aa |
| 活性数据 | MVTTLGPKMAAEWGGGVGYSGSGPGRSRWRWSGSVWVRSVLLLLGGLRASATSTPVSLGSSPPCRHHVPSDTEVINKVHLKANHVVKRDVDEHLRIKTVYDKSVEELLPEKKNLVKNKLFPQAISYLEKTFQVRRPAGTILLSRQCATNQYLRKENDPHRYCTGECAAHTKCGPVIVPEEHLQQCRVYRGGKWPHGAVGVPDQEGISDADFVLYVGALATERCSHENIISYAAYCQQEANMDRPIAGYANLCPNMISTQPQEFVGMLSTVKHEVIHALGFSAGLFAFYHDKDGNPLTSRFADGLPPFNYSLGLYQWSDKVVRKVERLWDVRDNKIVRHTVYLLVTPRVVEEARKHFDCPVLEGMELENQGGVGTELNHWEKRLLENEAMTGSHTQNRVLSRITLALMEDTGRQMLSPYCDTLRSNPLQLTCRQDQRAVAVCNLQKFPKPLPQEYQYFDELSGIPAEDLPYYGGSVEIADYCPFSQEFSWHLSGEYQRSSDCRILENQPEIFKNYGAEKYGPHSVCLIQKSAFVMEKCERKLSYPDWGSGCYQVSCSPQGLKVWVQDTSYLCSRAGQVLPVSIQMNGWIHDGNLLCPSCWDFCELCPPETDPPATNLTRALPLDLCSCSSSLVVTLWLLLGNLFPLLAGFLLCIWH |
| 分子量 | 99 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LMLN蛋白(假定为溶酶体相关膜蛋白或类似结构域蛋白)的假设性参考文献示例，基于常见重组蛋白研究的典型方向概括。实际文献需通过学术数据库检索确认：
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1. **文献名称**：*Expression and functional characterization of recombinant human LMLN in cancer cell invasion*
**作者**：Zhang Y. et al.
**摘要**：研究通过大肠杆菌系统表达重组人LMLN蛋白，验证其促进肿瘤细胞迁移和侵袭的功能，并发现其通过调控基质金属蛋白酶(MMPs)活性参与肿瘤转移。
2. **文献名称**：*Structural analysis of LMLN reveals a novel role in lysosomal enzyme trafficking*
**作者**：Tanaka K. et al.
**摘要**：利用X射线晶体学解析重组人LMLN蛋白的三维结构，揭示其与溶酶体酶运输相关的特异性结构域，为溶酶体贮积症提供潜在治疗靶点。
3. **文献名称**：*Development of a high-yield purification method for recombinant LMLN in mammalian cells*
**作者**：Lee S. et al.
**摘要**：优化哺乳动物细胞表达系统(如CHO细胞)，实现重组人LMLN蛋白的高效分泌表达及亲和层析纯化，确保蛋白的正确糖基化修饰。
4. **文献名称**：*LMLN as a biomarker for neurodegenerative diseases: insights from recombinant protein-based assays*
**作者**：Müller R. et al.
**摘要**：通过重组人LMLN蛋白开发ELISA检测方法，发现其在阿尔茨海默病患者脑脊液中的异常表达水平，提示其作为疾病标志物的潜力。
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**注意**：以上为模拟示例，实际文献需根据LMLN蛋白的具体定义(如基因符号全称)通过PubMed、Web of Science等平台检索。若“LMLN”存在拼写误差或为罕见蛋白，建议核对基因名称或补充更多背景信息。
Recombinant human LMLN (leucine-rich repeat and Malectin domain-containing protein) is a protein of growing interest in biomedical research due to its potential roles in cellular processes and disease pathways. LMLN, also known as malectin, is a conserved endoplasmic reticulum (ER)-resident protein that interacts with ribosome-bound oligosaccharyltransferases, playing a critical role in N-glycosylation quality control. It selectively recognizes and binds to misfolded glycoproteins, targeting them for ER-associated degradation (ERAD). This molecular chaperone function positions LMLN as a key regulator of protein homeostasis, with implications in diseases linked to protein misfolding, such as neurodegenerative disorders and cancer.
The recombinant form of human LMLN is typically produced using eukaryotic expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. Its recombinant production enables structural studies, interaction analyses, and functional investigations into ER stress responses. Recent studies suggest LMLN's involvement in autophagy, immune regulation, and cancer cell survival under stress conditions, making it a potential therapeutic target. Researchers utilize recombinant LMLN to develop screening platforms for drug candidates aiming to modulate ERAD pathways or alleviate proteostasis-related pathologies. Ongoing research continues to unravel its dual roles in both protein quality control and pathological mechanisms.
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