| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN7A |
| Uniprot No | O14910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233aa |
| 活性数据 | MLKPSVTSAP TADMATLTVV QPLTLDRDVA RAIELLEKLQ ESGEVPVHKL QSLKKVLQSE FCTAIREVYQ YMHETITVNG CPEFRARATA KATVAAFAAS EGHSHPRVVE LPKTDEGLGF NVMGGKEQNS PIYISRIIPG GVAERHGGLK RGDQLLSVNG VSVEGEHHEK AVELLKAAKD SVKLVVRYTP KVLEEMEARF EKLRTARRRQ QQQLLIQQQQ QQQQQQTQQN HMS |
| 分子量 | 25.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是围绕重组人LIN7A蛋白相关研究主题的3篇虚构参考文献案例(合理推测领域研究方向)：
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1. **文献名称**：*"Crystal structure of human LIN7A PDZ domain in complex with β-catenin reveals polarity complex assembly"*
**作者**：Joosten, R.P. et al.
**摘要**：本研究解析了重组人LIN7A蛋白PDZ结构域的晶体结构，发现其通过特异性结合β-catenin的C端基序调控细胞极性复合体组装，为上皮细胞极性缺失相关的癌症转移机制提供结构基础。
2. **文献名称**：*"LIN7A regulates synaptic GluA1 trafficking via recombinant protein interaction assays in neuronal cultures"*
**作者**：Zhang, Y. et al.
**摘要**：通过表达重组人LIN7A蛋白并进行体外互作实验，证明其与AMPA受体亚基GluA1的C端结合，调控突触后膜受体聚类，提示LIN7A在神经突触可塑性中的关键作用。
3. **文献名称**：*"Recombinant LIN7A expression in HEK293 cells facilitates analysis of basolateral membrane targeting in epithelial models"*
**作者**：Lee, S. & Nelson, W.J.
**摘要**：利用重组LIN7A蛋白在HEK293细胞中过表达，结合免疫荧光技术，揭示LIN7A通过与PAR3/PAR6/aPKC复合物协作，驱动细胞极性建立及膜蛋白定向运输的分子路径。
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**备注**：以上案例为模拟文献摘要逻辑，实际文献需通过PubMed/Google Scholar检索关键词(如LIN7A + recombinant, LIN7A structural analysis, LIN7A interaction partners)获取。真实研究方向多涉及细胞极性、神经突触、受体运输及癌症生物学领域。
Recombinant human LIN7A protein is a key scaffold protein involved in cell polarity, synaptic organization, and signal transduction. LIN7A (also known as Veli-1 or MALS-1) belongs to the LIN7 family and interacts with PDZ domain-containing proteins, including PSD-95 and CASK, to regulate membrane protein trafficking and maintain cell polarity. It plays a critical role in epithelial cell tight junction formation by stabilizing the Crumbs-PALS1-PATJ polarity complex. LIN7A also facilitates neurotransmitter receptor clustering at synapses and modulates intracellular signaling through interactions with β-catenin and cytoskeletal components.
Recombinant LIN7A is typically expressed in bacterial or mammalian systems for functional studies, enabling researchers to investigate its structural domains, binding partners, and regulatory mechanisms. Its recombinant form is essential for exploring diseases linked to polarity defects, such as cancer metastasis, neurological disorders (e.g., autism, schizophrenia), and epithelial barrier dysfunction. Studies using purified LIN7A help elucidate its conformational changes during complex assembly and evaluate therapeutic strategies targeting cell polarity pathways.
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