| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN28 |
| Uniprot No | Q9H9Z2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 活性数据 | MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN |
| 分子量 | 49.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"LIN28: A regulator of cell fate and a target for cellular reprogramming" by Yu et al., 2007**
- 研究LIN28在体细胞重编程中的作用，证实重组人LIN28蛋白可协同其他转录因子(如OCT4、SOX2)将体细胞转化为诱导多能干细胞(iPSCs)，并揭示其通过抑制let-7 miRNA促进细胞多能性。
2. **"Structural basis for recognition of selectively modified RNA by LIN28" by Zhu et al., 2013**
- 解析重组人LIN28蛋白与RNA结合的晶体结构，阐明其通过冷休克结构域(CSD)和锌指结构域优先结合富含尿嘧啶的RNA序列，揭示其在调控let-7加工中的分子机制。
3. **"LIN28 promotes tumor progression via let-7-dependent and -independent mechanisms in cancer" by Viswanathan et al., 2009**
- 利用重组LIN28蛋白进行功能实验，发现其通过抑制let-7 miRNA促进癌细胞增殖和转移，同时独立于let-7激活致癌信号通路(如mTOR)，揭示其在癌症中的双重作用。
4. **"Recombinant LIN28 regulates metabolic reprogramming in human pluripotent cells" by Nguyen et al., 2016**
- 通过体外实验证明，重组人LIN28蛋白可增强人干细胞的糖酵解代谢，促进其自我更新，相关机制涉及对线粒体代谢基因的转录后调控，表明LIN28在代谢与多能性间的关键联系。
(注：以上文献信息为示例，实际引用需核实原文准确性。)
LIN28 is a highly conserved RNA-binding protein playing pivotal roles in embryonic development, pluripotency, and cellular reprogramming. Originally identified in *Caenorhabditis elegans* as a regulator of developmental timing, human LIN28 exists as two paralogs: LIN28A (abundant in embryonic stem cells) and LIN28B (primarily expressed during embryogenesis). Both function through post-transcriptional regulation, notably by binding to mRNAs and microRNAs, particularly blocking the biogenesis of let-7 tumor suppressor miRNAs. This interaction influences metabolic pathways, stem cell renewal, and somatic cell reprogramming.
Recombinant human LIN28 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce functional, purified protein for research. Its structure includes cold-shock domains and zinc-knuckle motifs critical for RNA interactions. Studies leveraging recombinant LIN28 have elucidated its role in maintaining stem cell pluripotency, promoting tissue regeneration, and driving oncogenesis when dysregulated. Overexpression correlates with aggressive cancers (e.g., liver, ovarian) and poor prognosis, positioning LIN28 as both a therapeutic target and a biomarker.
Research applications span regenerative medicine (enhancing induced pluripotent stem cell generation), cancer biology, and developmental studies. However, challenges remain in deciphering its context-dependent dual roles—supporting normal development versus fueling malignancy. Current efforts focus on modulating LIN28-let-7 interactions for therapeutic intervention, highlighting its significance in both basic science and translational medicine.
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