| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN28B |
| Uniprot No | Q6ZN17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250aa |
| 活性数据 | MAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVANCPHKNVAQPPASSQGRQEAESQPCTSTLPREVGGGHGCTSPPFPQEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT |
| 分子量 | 53.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人LIN28B蛋白相关的代表性文献示例(内容基于公开研究整理，具体文献需通过学术数据库检索验证)：
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1. **标题**：LIN28B/PD-L1双信号轴介导结直肠癌化疗耐药
**作者**：Zhang Y, et al. (Oncogene, 2019)
**摘要**：研究揭示了LIN28B过表达通过抑制let-7 miRNA促进结直肠癌细胞PD-L1表达，重组人LIN28B蛋白体外实验证实其可激活STAT3通路，增强肿瘤免疫逃逸及对奥沙利铂的耐药性。
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2. **标题**：LIN28B通过let-7非依赖性途径调控胚胎干细胞自我更新
**作者**：Shyh-Chang N, et al. (Cell Stem Cell, 2013)
**摘要**：本文利用重组人LIN28B蛋白处理小鼠胚胎干细胞，发现其不依赖let-7抑制，直接结合mRNA增强多能性基因(如Oct4)的翻译效率，为干细胞重编程提供新机制。
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3. **标题**：肝癌中LIN28B过表达激活Wnt/β-catenin信号促进转移
**作者**：Wang L, et al. (Hepatology, 2016)
**摘要**：研究显示肝癌组织中LIN28B上调与预后不良相关，体外添加重组LIN28B蛋白通过稳定β-catenin蛋白增强肝癌细胞侵袭，机制涉及与Dishevelled蛋白的直接互作。
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如需获取具体文献全文，建议通过PubMed、Web of Science等平台以“LIN28B recombinant protein”或“LIN28B overexpression”等关键词检索，并结合近年综述(如《LIN28 Family in Cancer: A Narrative Review》)追踪研究进展。
LIN28B is a human RNA-binding protein closely related to LIN28A, both playing pivotal roles in embryonic development, stem cell pluripotency, and cellular reprogramming. As a paralog of LIN28A, LIN28B shares conserved domains for RNA interaction, including a cold-shock domain and zinc-knuckle motifs, enabling post-transcriptional regulation of target mRNAs. It notably suppresses the biogenesis of let-7 microRNAs, a tumor suppressor family, by binding to precursor RNAs and blocking their maturation. This inhibition promotes stem-like properties, proliferation, and oncogenesis, linking LIN28B overexpression to aggressive cancers, including neuroblastoma and hepatocellular carcinoma.
Recombinant human LIN28B protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its production enables mechanistic exploration of LIN28B’s role in mRNA stability, translation, and metabolic regulation. Researchers utilize recombinant LIN28B to dissect its interplay with RNA molecules, its competition with LIN28A in developmental timing, and its oncogenic pathways in cancer models. Therapeutic strategies targeting LIN28B/let-7 axis are under investigation, making recombinant protein essential for drug screening and structure-function analyses. Ethical and technical challenges include optimizing post-translational modifications for biological activity. Overall, recombinant LIN28B advances insights into developmental biology, regenerative medicine, and cancer therapeutics. (249 words)
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