| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIMK2 |
| Uniprot No | P53671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-686aa |
| 活性数据 | MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK |
| 分子量 | 101 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LIMK2蛋白的模拟参考文献示例(仅供学术参考，建议通过数据库核实真实文献)：
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1. **标题**: *"Expression and functional characterization of recombinant human LIMK2 in neuronal cells"*
**作者**: Zhang, Y. et al.
**摘要**: 研究报道了通过大肠杆菌系统成功表达并纯化重组人LIMK2蛋白，验证其在神经元细胞中通过磷酸化cofilin调节肌动蛋白动态，可能参与阿尔茨海默病中tau蛋白异常聚集的病理机制。
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2. **标题**: *"Structural insights into LIMK2 activation by Rho signaling pathways"*
**作者**: Patel, R., & Kumar, S.
**摘要**: 通过X射线晶体学解析了重组LIMK2蛋白的活性构象，揭示其被Rho/ROCK通路激活的分子机制，并提出靶向LIMK2的构象调控可能抑制癌症细胞迁移。
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3. **标题**: *"LIMK2 as a potential therapeutic target in breast cancer metastasis"*
**作者**: Lee, H. et al.
**摘要**: 利用重组LIMK2蛋白进行体外实验，发现其过表达促进乳腺癌细胞侵袭，通过抑制cofilin磷酸化可阻断肿瘤转移，表明LIMK2是潜在抗转移治疗靶点。
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4. **标题**: *"Development of a high-yield purification protocol for recombinant human LIMK2 and its kinase activity profiling"*
**作者**: Garcia, M. et al.
**摘要**: 提出一种高效的重组LIMK2蛋白杆状病毒表达系统，优化纯化步骤获得高活性蛋白，并通过激酶谱分析其底物特异性，为药物筛选提供工具。
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**提示**：以上为模拟文献，实际研究请通过PubMed、Web of Science或Google Scholar检索关键词“recombinant LIMK2”、“LIMK2 expression/function”等获取。
Recombinant human LIMK2 protein is a genetically engineered form of the LIM domain kinase 2 (LIMK2), a serine/threonine kinase belonging to the LIM kinase family. LIMK2 plays a crucial role in regulating actin cytoskeleton dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This kinase is involved in diverse cellular processes, including cell motility, division, and differentiation, primarily through Rho GTPase signaling pathways (e.g., RhoA/ROCK and Rac/PAK). Dysregulation of LIMK2 has been implicated in pathologies such as cancer metastasis, neurological disorders (e.g., Alzheimer’s disease), and developmental defects.
The recombinant form is produced using expression systems like *E. coli* or mammalian cell lines, enabling high-purity, active protein for research. Its production facilitates studies on LIMK2's structure-function relationships, substrate interactions, and signaling mechanisms. Researchers use it to screen inhibitors for therapeutic development, particularly targeting cancer invasion or neurodegenerative pathways. Compared to endogenous LIMK2. the recombinant protein offers controlled experimental conditions, reducing variability from cellular contexts. Current research also explores its role in synaptic plasticity and tau phosphorylation, linking it to neurodegenerative disease models. Structural studies focus on its N-terminal LIM domains (mediating protein interactions) and C-terminal kinase domain, aiding drug design efforts.
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