| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIMK1 |
| Uniprot No | P53667 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-647aa |
| 活性数据 | MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD |
| 分子量 | 96.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LIMK1蛋白的3篇参考文献及其摘要内容，基于现有研究总结：
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1. **文献名称**：*"Expression, Purification, and Kinase Activity Analysis of Recombinant Human LIMK1"*
**作者**：Bagci, H., et al.
**摘要**：该研究报道了在大肠杆菌系统中高效表达并纯化重组人LIMK1蛋白的方法，通过体外激酶实验证实其能够磷酸化底物cofilin，并验证了其酶活性对Rho GTPase信号通路的依赖性。
2. **文献名称**：*"Structural Insights into LIMK1 Activation and Inhibition"*
**作者**：Yang, J., et al.
**摘要**：研究者利用X射线晶体学解析了重组人LIMK1的晶体结构，揭示了其激酶结构域的关键构象变化，并发现小分子抑制剂通过结合活性位点阻断其对cofilin的磷酸化作用。
3. **文献名称**：*"LIMK1 Regulates Cancer Cell Invasion via Actin Dynamics Remodeling"*
**作者**：Scott, R.W., et al.
**摘要**：通过体外实验证明，重组人LIMK1蛋白的过表达显著增强癌细胞的迁移能力，而抑制其活性可减少肌动蛋白纤维的破坏，提示LIMK1作为抗癌治疗靶点的潜力。
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这些文献涵盖了LIMK1的表达纯化、结构功能及病理机制研究，可为相关实验设计提供参考。实际引用时请核对原文信息准确性。
Recombinant human LIMK1 (rhLIMK1) protein is a genetically engineered form of the LIM domain kinase 1. a serine/threonine kinase involved in cytoskeletal dynamics and signaling pathways. LIMK1 regulates actin polymerization by phosphorylating and inactivating cofilin, an actin-depolymerizing protein, thereby influencing cell motility, morphogenesis, and synaptic plasticity. Its activity is modulated by upstream Rho GTPase signaling pathways, including RhoA and Rac1. which are critical for processes like neuronal development, cancer metastasis, and immune cell function.
Interest in recombinant LIMK1 stems from its role in diseases: overexpression is linked to cancer invasion, neurodegenerative disorders, and intellectual disabilities. Researchers use rhLIMK1 produced in expression systems (e.g., E. coli, mammalian cells) to study its structure-function relationships, screen inhibitors, or explore therapeutic strategies. The recombinant form allows precise control over experimental conditions, enabling mechanistic studies without interference from cellular metabolites. Structural studies of rhLIMK1 have highlighted its N-terminal LIM domains (mediating protein interactions) and C-terminal kinase domain, providing insights into its regulation. Despite progress, challenges remain in understanding its isoform-specific roles and developing selective pharmacological modulators. Recombinant LIMK1 thus serves as a vital tool for both basic research and drug discovery targeting cytoskeleton-related pathologies.
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