| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIG3 |
| Uniprot No | P49916 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 活性数据 | MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGGVA |
| 分子量 | 60 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LIG3蛋白的**模拟参考文献示例**，供学术写作格式参考。建议通过PubMed或Google Scholar查找真实文献：
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1. **文献名称**: *Structural Basis of Human DNA Ligase III Recognition of DNA Ends*
**作者**: Cotner-Gohara, E. et al.
**摘要**: 通过X射线晶体学解析重组人LIG3蛋白的催化核心结构，揭示其通过动态构象变化识别DNA断端并催化连接的分子机制，为DNA修复途径提供结构生物学依据。
2. **文献名称**: *XRCC1 Coordinates Activity of DNA Ligase IIIα in Base Excision Repair*
**作者**: Caldecott, K.W. et al.
**摘要**: 研究证明LIG3与XRCC1蛋白的BRCT结构域相互作用，形成复合物以增强碱基切除修复效率，阐明其在基因组稳定性中的关键作用。
3. **文献名称**: *Mitochondrial DNA Repair by Ligase III Dependency in Mammalian Cells*
**作者**: Simsek, D. et al.
**摘要**: 发现重组人LIG3蛋白在线粒体中的表达对维持mtDNA完整性至关重要，缺失导致线粒体功能障碍，提示其与衰老及神经退行性疾病的关联。
4. **文献名称**: *Functional Characterization of Recombinant Human LIG3 in Single-Strand Break Repair*
**作者**: Moser, J. et al.
**摘要**: 体外实验验证重组LIG3蛋白修复单链DNA断裂的能力，证明其与PARP1的协同作用及在癌症化疗耐药中的潜在影响。
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**注意**：以上内容为模拟生成，非真实文献。建议通过学术数据库检索关键词(如“recombinant human LIG3”、“DNA ligase III repair”)获取最新研究。
**Background of Recombinant Human LIG3 Protein**
Recombinant human DNA ligase III (LIG3) is a critical enzyme involved in DNA repair pathways, primarily the base excision repair (BER) and alternative non-homologous end joining (NHEJ). LIG3. encoded by the *LIG3* gene, functions in both the nucleus and mitochondria, playing a unique role in maintaining genomic and mitochondrial DNA stability. Unlike other DNA ligases, LIG3 interacts with the scaffolding protein XRCC1 to coordinate single-strand break repair and acts as a backup ligase in double-strand break repair when DNA ligase IV is unavailable. Its mitochondrial isoform is essential for repairing oxidative damage in mitochondrial DNA.
The recombinant form of LIG3 is produced via genetic engineering in expression systems (e.g., *E. coli* or mammalian cells*)*, ensuring high purity and activity for experimental applications. Studies utilize recombinant LIG3 to investigate its enzymatic mechanisms, interactions with repair partners, and its role in diseases like cancer and neurodegenerative disorders. Notably, mutations or dysregulation of LIG3 are linked to chemotherapy resistance and mitochondrial dysfunction, highlighting its therapeutic potential. By enabling biochemical and structural analyses, recombinant LIG3 accelerates research into DNA repair pathways and targeted drug development.
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