| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LHFPL5 |
| Uniprot No | Q8TAF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249aa |
| 活性数据 | MVKLLPAQEAAKIYHTNYVRNSRAVGVMWGTLTICFSVLVMALFIQPYWIGDSVNTPQAGYFGLFSYCVGNVLSSELICKGGPLDFSSIPSRAFKTAMFFVALGMFLIIGSIICFSLFFICNTATVYKICAWMQLAAATGLMIGCLVYPDGWDSSEVRRMCGEQTGKYTLGHCTIRWAFMLAILSIGDALILSFLAFVLGYRQDKLLPDDYKADGTEEV |
| 分子量 | 50.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LHFPL5蛋白的3条模拟参考文献示例(基于研究领域知识总结，非真实文献)：
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1. **文献名称**：*LHFPL5 interacts with transmembrane channel-like proteins to regulate auditory mechanotransduction*
**作者**：Kalappa BI, et al.
**摘要**：该研究通过免疫共沉淀和质谱分析，揭示了人LHFPL5蛋白与跨膜通道样蛋白TMC1/TMC2的相互作用，证明其在耳蜗毛细胞机械转导通道复合体组装中的关键作用。重组人LHFPL5蛋白的表达实验进一步支持其维持静纤毛结构和信号传递的功能。
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2. **文献名称**：*Expression and purification of recombinant human LHFPL5 in HEK293 cells for functional studies*
**作者**：Wang Y, et al.
**摘要**：报道了一种利用哺乳动物HEK293细胞高效表达重组人LHFPL5蛋白的方法，通过His标签亲和层析纯化获得高纯度蛋白。生物物理特性分析表明该蛋白具有稳定的折叠结构，可结合脂质膜组分，为体外研究其分子机制提供了工具。
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3. **文献名称**：*Targeted disruption of Lhfpl5 in mice leads to progressive hearing loss and hair cell degeneration*
**作者**：Longo-Guess CM, et al.
**摘要**：构建了Lhfpl5基因敲除小鼠模型，发现其表现出渐进性听力丧失和毛细胞退化。通过注射重组人LHFPL5蛋白到内耳，部分恢复了幼鼠的听觉功能，提示其在治疗遗传性耳聋中的潜在应用价值。
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注：上述文献为示例性内容，实际引用时需检索真实数据库(如PubMed)获取准确信息。
Recombinant human LHFPL5 (Lipoma HMGIC Fusion Partner-Like 5) protein is a key research target in hearing-related studies due to its critical role in auditory function. LHFPL5. a transmembrane protein predominantly expressed in the inner ear, is essential for the development and maintenance of hair cell stereocilia—specialized structures vital for mechanoelectrical transduction in hearing. Mutations in the LHFPL5 gene are linked to autosomal recessive nonsyndromic hearing loss (DFNB67), highlighting its importance in auditory physiology.
The recombinant form of LHFPL5 is typically produced using mammalian or insect expression systems to ensure proper post-translational modifications. It consists of four transmembrane domains and cytoplasmic N- and C-termini, structurally resembling other tetraspanin-like proteins involved in protein interactions. Studies employ recombinant LHFPL5 to investigate its interaction partners (e.g., TMC1. a component of mechanotransduction channels) and its role in organizing hair bundle architecture.
Research applications include elucidating molecular mechanisms of hereditary deafness, screening potential therapeutic compounds, and developing gene-editing strategies. Recombinant LHFPL5 also aids in structural studies using cryo-EM or X-ray crystallography to map functional domains. Its production enables antibody development for diagnostic tools and validation of disease-associated mutations.
Overall, recombinant LHFPL5 serves as a vital tool for decoding auditory signal transduction pathways and advancing therapies for hearing disorders.
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