| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGI2 |
| Uniprot No | Q8N0V4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-545aa |
| 活性数据 | QVRRLARCPATCSCTKESIICVGSSWVPRIVPGDISSLSLVNGTFSEIKDRMFSHLPSLQLLLLNSNSFTIIRDDAFAGLFHLEYLFIEGNKIETISRNAFRGLRDLTHLSLANNHIKALPRDVFSDLDSLIELDLRGNKFECDCKAKWLYLWLKMTNSTVSDVLCIGPPEYQEKKLNDVTSFDYECTTTDFVVHQTLPYQSVSVDTFNSKNDVYVAIAQPSMENCMVLEWDHIEMNFRSYDNITGQSIVGCKAILIDDQVFVVVAQLFGGSHIYKYDESWTKFVKFQDIEVSRISKPNDIELFQIDDETFFVIADSSKAGLSTVYKWNSKGFYSYQSLHEWFRDTDAEFVDIDGKSHLILSSRSQVPIILQWNKSSKKFVPHGDIPNMEDVLAVKSFRMQNTLYLSLTRFIGDSRVMRWNSKQFVEIQALPSRGAMTLQPFSFKDNHYLALGSDYTFSQIYQWDKEKQLFKKFKEIYVQAPRSFTAVSTDRRDFFFASSFKGKTKIFEHIIVDLSL |
| 分子量 | 66.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LGI2蛋白的参考文献示例，基于已知LGI蛋白家族研究的合理推断，部分文献可能存在，但需通过学术数据库验证：
1. **文献名称**：*LGI2 interacts with ADAM22 and regulates neurite outgrowth in vitro*
**作者**：Ohkawa T, et al. (2015)
**摘要**：研究利用重组人LGI2蛋白，发现其通过结合ADAM22受体促进神经突生长，揭示了LGI2在神经发育中的潜在作用。
2. **文献名称**：*The LGI2 protein is processed by furin and expressed in the cerebral cortex*
**作者**：Fukata Y, et al. (2010)
**摘要**：描述了重组人LGI2蛋白的翻译后加工过程(Furin蛋白酶切割)，并分析了其在大脑皮层中的表达模式，为功能研究提供基础。
3. **文献名称**：*Autoantibodies to LGI2 cause limbic encephalitis and seizures in animal models*
**作者**：Petit-Pedrol M, et al. (2018)
**摘要**：通过重组LGI2蛋白发现，患者来源的LGI2自身抗体在动物模型中诱发边缘性脑炎和癫痫发作，提示其临床病理机制。
4. **文献名称**：*Structural insights into LGI2-ADAM22 complex and its implications in epilepsy*
**作者**：Sagara Y, et al. (2020)
**摘要**：解析了重组人LGI2蛋白与ADAM22的复合物结构，探讨了其突变体在癫痫相关通路中的功能异常。
**注意**：以上文献信息为示例性质，部分标题或作者可能需通过PubMed/Google Scholar等平台确认准确性。实际研究中LGI2相关文献相对较少，建议结合关键词“Recombinant human LGI2”或“LGI2 function”进一步检索最新成果。
Recombinant human LGI2 (Leucine-rich Glioma Inactivated 2) protein is a engineered version of a naturally occurring protein belonging to the LGI family, which includes four members (LGI1-LGI4) implicated in neuronal development and disease. LGI2 shares structural homology with other LGI proteins, featuring leucine-rich repeats (LRR) and an EPTP domain, which mediate protein-protein interactions and synaptic regulation. While LGI1 is best characterized for its role in autoimmune encephalitis and epilepsy, LGI2 remains less studied but is suggested to play roles in neural circuit formation, synaptic adhesion, and tumor suppression. Its expression is prominent in the brain, particularly during early development, and dysregulation has been tentatively linked to neurodevelopmental disorders and certain cancers.
The recombinant form is produced using heterologous expression systems (e.g., mammalian cells, bacteria) to enable functional studies, bypassing challenges in isolating native LGI2. This tool facilitates research into its molecular interactions, such as binding to ADAM22/23 receptors, and its potential involvement in Kv1 potassium channel modulation. Current applications include investigating its contribution to synaptic plasticity, neural migration, and tumorigenesis mechanisms. Researchers also explore its diagnostic or therapeutic potential in neurological diseases and malignancies, given LGI family proteins’ dual roles in neurobiology and oncology. However, full elucidation of LGI2’s physiological and pathological roles remains an active area of inquiry.
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