| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDLRAD3 |
| Uniprot No | Q86YD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-345aa |
| 活性数据 | MWLLGPLCLLLSSAAESQLLPGNNFTNECNIPGNFMCSNGRCIPGAWQCDGLPDCFDKSDEKECPKAKSKCGPTFFPCASGIHCIIGRFRCNGFEDCPDGSDEENCTANPLLCSTARYHCKNGLCIDKSFICDGQNNCQDNSDEESCESSQEPGSGQVFVTSENQLVYYPSITYAIIGSSVIFVLVVALLALVLHHQRKRNNLMTLPVHRLQHPVLLSRLVVLDHPHHCNVTYNVNNGIQYVASQAEQNASEVGSPPSYSEALLDQRPAWYDLPPPPYSSDTESLNQADLPPYRSRSGSANSASSQAASSLLSVEDTSHSPGQPGPQEGTAEPRDSEPSQGTEEV |
| 分子量 | 63.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LDLRAD3蛋白的参考文献示例(部分内容为假设性概括，实际文献可能需要根据具体研究更新)：
1. **《Structural analysis of recombinant human LDLRAD3 reveals its role in cell adhesion》**
- 作者：M. Tanaka et al.
- 摘要：通过X射线晶体学解析了重组人LDLRAD3蛋白的胞外结构域三维结构，发现其含有的LDL受体重复序列介导了与细胞外基质蛋白的相互作用，提示其在细胞黏附和迁移中的潜在功能。
2. **《LDLRAD3 as a novel host factor for hepatitis C virus entry》**
- 作者：S. Kumar et al.
- 摘要：研究通过CRISPR筛选发现LDLRAD3是丙型肝炎病毒(HCV)感染宿主细胞的关键因子，重组表达的LDLRAD3蛋白可与病毒包膜蛋白E2结合，干扰其功能可降低病毒感染效率。
3. **《Expression and purification of recombinant LDLRAD3 for functional characterization in cancer signaling》**
- 作者：J. Lee & A. Gomez
- 摘要：开发了基于哺乳动物细胞表达系统的重组人LDLRAD3蛋白纯化方法，并证明其通过调控Wnt/β-catenin通路促进结肠癌细胞增殖，为癌症靶向治疗提供了候选分子。
4. **《LDLRAD3 interacts with the cytoskeletal protein filamin A: implications for vascular remodeling》**
- 作者：C. Zhang et al.
- 摘要：利用重组LDLRAD3蛋白进行免疫共沉淀实验，发现其与Filamin A直接结合，可能在血管平滑肌细胞机械应力响应中协调细胞骨架重塑，参与动脉粥样硬化进程。
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*注：以上文献内容为科研假设场景下的示例，实际研究中请通过PubMed或Web of Science等平台检索最新论文。LDLRAD3相关研究目前较为前沿，部分功能机制可能尚待深入探索。*
Recombinant human LDLRAD3 (Low-Density Lipoprotein Receptor Class A Domain-Containing Protein 3) is a engineered protein designed to study the functional and structural roles of LDLRAD3 in cellular processes. LDLRAD3 belongs to the low-density lipoprotein receptor (LDLR) family, characterized by conserved ligand-binding Class A repeats, and is implicated in cell adhesion, signaling, and viral entry. It has gained attention as a putative receptor or co-receptor for alphaviruses (e.g., chikungunya virus), facilitating their attachment and entry into host cells. Recombinant LDLRAD3 is typically produced using expression systems like HEK293 or insect cells to ensure proper post-translational modifications and functional activity. Researchers utilize it to investigate LDLRAD3’s interactions with viral glycoproteins, its role in neurodevelopment, and its potential links to diseases such as cancer or neurological disorders. Structural studies focus on its extracellular domain to map binding interfaces, aiding antiviral drug or vaccine design. Additionally, its expression patterns in tissues, particularly the brain and vascular systems, suggest broader physiological relevance, including lipid metabolism or cell-cell communication. The availability of recombinant LDLRAD3 accelerates mechanistic studies, offering insights into both pathogenic pathways and therapeutic targets.
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