| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD2AP |
| Uniprot No | Q9Y5K6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MVDYIVEYDY DAVHDDELTI RVGEIIRNVK KLQEEGWLEG ELNGRRGMFP DNFVKEIKRE TEFKDDSLPI KRERHGNVAS LVQRISTYGL PAGGIQPHPQ TKNIKKKTKK RQCKVLFEYI PQNEDELELK VGDIIDINEE VEEGWWSGTL NNKLGLFPSN FVKELEVTDD GETHEAQDDS ETVLAGPTSP IPSLGNVSET ASGSVTQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK PEKPLILQSL GPKTQSVEIT KTDTEGKIKA KEYCRTLFAY EGTNEDELTF KEGEIIHLIS KETGEAGWWR GELNGKEGVF PDNFAVQINE LDKDFPKPKK PPPPAKAPAP KPELIAAEKK YFSLKPEEKD EKSTLEQKPS KPAAPQVPPK KPTPPTKASN LLRSSGTVYP KRPEKPVPPP PPIAKINGEV SSISSKFETE PVSKLKLDSE QLPLRPKSVD FDSLTVRTSK ETDVVNFDDI ASSENLLHLT ANRPKMPGRR LPGRFNGGHS PTHSPEKILK LPKEEDSANL KPSELKKDTC YSPKPSVYLS TPSSASKANT TAFLTPLEIK AKVETDDVKK NSLDELRAQI IELLCIVEAL KKDHGKELEK LRKDLEEEKT MRSNLEMEIE KLKKAVLSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD2AP重组蛋白的3篇参考文献，涵盖其结构、功能及疾病相关研究：
1. **文献名称**：*Structural and functional analysis of the CD2AP proline-rich domain*
**作者**：Smith A, et al.
**摘要**：通过重组表达CD2AP的富含脯氨酸结构域，研究其与细胞骨架蛋白(如肌动蛋白)的相互作用，揭示其在细胞迁移中的调控机制。
2. **文献名称**：*CD2AP interacts with nephrin to maintain glomerular filtration barrier integrity*
**作者**：Jones B, et al.
**摘要**：利用重组CD2AP蛋白验证其与nephrin的相互作用，证实该复合物在肾小球滤过屏障中的关键作用，为肾病机制提供分子基础。
3. **文献名称**：*CD2AP deficiency promotes tumor growth via EGFR signaling in colorectal cancer*
**作者**：Wang C, et al.
**摘要**：通过体外重组CD2AP蛋白实验，发现其缺失导致EGFR信号通路异常激活，促进结直肠癌细胞增殖和转移。
4. **文献名称**：*Recombinant CD2AP SH3 domains as inhibitors of amyloid-β aggregation in Alzheimer's disease*
**作者**：Zhang L, et al.
**摘要**：研究CD2AP的SH3结构域重组蛋白对β淀粉样蛋白聚集的抑制作用，提示其在阿尔茨海默病治疗中的潜在应用价值。
(注：以上文献为示例性内容，实际引用需以具体论文为准。)
CD2-associated protein (CD2AP) is a cytosolic adaptor protein first identified for its role in stabilizing the interaction between CD2 (a cell surface receptor on T cells) and its ligand CD58. facilitating immune synapse formation during T-cell activation. Structurally, CD2AP is a ~60 kDa protein containing three Src homology 3 (SH3) domains and a coiled-coil domain, enabling interactions with multiple signaling and structural proteins. Beyond immunology, CD2AP is critical in maintaining renal filtration barriers by anchoring podocyte foot processes to the glomerular basement membrane. Knockout mice lacking CD2AP develop severe proteinuria and kidney dysfunction, resembling human focal segmental glomerulosclerosis (FSGS), highlighting its essential role in podocyte integrity.
CD2AP also regulates endocytosis, vesicular trafficking, and cytoskeletal remodeling through partnerships with proteins like nephrin, cortactin, and CIN85. Its dysfunction is linked to Alzheimer’s disease, where disrupted endocytic pathways may promote amyloid-β accumulation, and to cancer progression due to altered cell migration and survival signaling. Recombinant CD2AP proteins—engineered via bacterial or mammalian expression systems—are widely used to study these mechanisms. Purified CD2AP aids in mapping protein interaction networks, probing structural-functional relationships, and screening therapeutic compounds targeting CD2AP-associated diseases. In nephrology research, recombinant CD2AP helps model podocyte injury and test interventions to restore filtration barrier function. Its versatility as a molecular tool continues to advance understanding of cellular dynamics in health and disease.
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