| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDHAL6B |
| Uniprot No | Q9BYZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 活性数据 | MSWTVPVVRASQRMSSVGANFLCLGMALCLRQATRIPLNGTWLFTPVSKMATVKSELIERFTSEKPVHHSKVSIIGTGSVGMACAISILLKGLSDELALVDLDEDKLKGETMDLQHGSPFTKMPNIVCSKDYFVTANSNLVIITAGARQEKGETRLNLVQRNVAIFKLMISSIVQYSPHCKLIIVSNPVDILTYVAWKLSAFPKNRIIGSGCNLDTARFRFLIGQKLGIHSESCHGWILGEHGDSSVPVWSGVNIAGVPLKDLNSDIGTDKDPEQWKNVHKEVTATAYEIIKMKGYTSWAIGLSVADLTESILKNLRRIHPVSTITKGLYGIDEEVFLSIPCILGENGITNLIKIKLTPEEEAHLKKSAKTLWEIQNKLKL |
| 分子量 | 68.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LDHAL6B蛋白的3篇示例性参考文献(注：文献信息仅为示例性描述，可能与实际研究存在差异)：
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1. **文献名称**：*LDHAL6B: A Testis-Specific Lactate Dehydrogenase with Distinct Enzymatic Properties*
**作者**：Smith J et al.
**摘要**：本研究首次报道了人LDHAL6B蛋白的重组表达及酶动力学特征。作者通过大肠杆菌系统成功纯化该蛋白，发现其在低氧条件下催化乳酸生成丙酮酸的活性显著高于其他LDH同工酶，提示其可能参与精子发生过程中的能量代谢调控。
2. **文献名称**：*Structural Insights into the Substrate Specificity of LDHAL6B*
**作者**：Li X et al.
**摘要**：通过X射线晶体学解析了重组人LDHAL6B的3D结构(分辨率2.1Å)，揭示了其底物结合口袋的独特氨基酸组成。实验表明，该蛋白对2-氧代丁酸具有高亲和力，可能与睾丸组织中的酮体代谢通路相关。
3. **文献名称**：*LDHAL6B Knockdown Impairs Sertoli Cell Glycolysis and Male Fertility*
**作者**：Wang Y et al.
**摘要**：利用CRISPR/Cas9构建LDHAL6B敲除小鼠模型，发现该蛋白缺失导致支持细胞糖酵解能力下降和精子发育异常。重组人LDHAL6B蛋白体外补充实验部分恢复代谢缺陷，证明其在雄性生殖系统中的关键作用。
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**说明**：LDHAL6B作为LDH-A亚基家族成员，现有研究多聚焦于其在生殖系统特异性表达及代谢调控功能，上述示例反映了当前该领域的研究热点方向。实际文献需通过PubMed或Web of Science以“LDHAL6B AND recombinant”为关键词检索获取。
Recombinant human LDHAL6B protein is a genetically engineered form of lactate dehydrogenase (LDH) subunit alpha-like 6B, a member of the LDH enzyme family that catalyzes the interconversion of pyruvate and lactate during glycolysis. LDHAL6B, located on the X chromosome, is a testis-specific isoform predominantly expressed in spermatogenic cells, suggesting a role in male germ cell metabolism and energy production during spermatogenesis. Unlike the widely expressed LDH-A (LDHA) and LDH-B (LDHB) isoforms, LDHAL6B exhibits restricted tissue distribution, underscoring its specialized function in sperm maturation and motility by regulating lactate levels in the testicular microenvironment.
The recombinant protein is typically produced in bacterial or mammalian expression systems, enabling studies on its enzymatic properties, structural characteristics, and interactions with other proteins. Its development has facilitated research into metabolic adaptations in germ cells, potential links to male infertility, and broader implications in energy metabolism. While the biological significance of LDHAL6B remains less characterized compared to other LDH isoforms, its unique expression pattern and role in anaerobic glycolysis make it a compelling target for reproductive biology and metabolic disorder research. Ongoing studies aim to clarify its regulatory mechanisms and therapeutic potential.
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