| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDHAL6A |
| Uniprot No | Q6ZMR3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332aa |
| 活性数据 | MATIKSELIK NFAEEEAIHH NKISIVGTGS VGVACAISIL LKGLSDELVL VDVDEGKLKG ETMDLQHGSP FMKMPNIVSS KDYLVTANSN LVIITAGARQ KKGETRLDLV QRNVSIFKLM IPNITQYSPH CKLLIVTNPV DILTYVAWKL SGFPKNRVIG SGCNLDSARF RYFIGQRLGI HSESCHGLIL GEHGDSSVPV WSGVNIAGVP LKDLNPDIGT DKDPEQWENV HKKVISSGYE MVKMKGYTSW GISLSVADLT ESILKNLRRV HPVSTLSKGL YGINEDIFLS VPCILGENGI TDLIKVKLTL EEEACLQKSA ETLWEIQKEL KL |
| 分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LDHAL6A蛋白的3篇文献简述(文献为模拟示例，实际引用需检索数据库验证)：
1. **"Cloning and enzymatic characterization of recombinant human LDHAL6A: implications in spermatogenesis"**
- **作者**: Wang, X. et al.
- **摘要**: 研究报道了LDHAL6A在睾丸组织中的特异性表达，通过在大肠杆菌中重组表达并纯化该蛋白，发现其对乳酸代谢的关键作用，提示其在精子形成中的潜在调控功能。
2. **"Structural basis for the isoform-specific inhibition of LDHAL6A in cancer metabolism"**
- **作者**: Kim, S. & Patel, R.
- **摘要**: 解析了重组人LDHAL6A的晶体结构，揭示了其与LDHA/B亚型的不同底物结合位点，并通过体外实验验证其在高通量抗癌药物筛选中的应用潜力。
3. **"Functional redundancy and divergence within the lactate dehydrogenase family: Role of LDHAL6A in hypoxia adaptation"**
- **作者**: Gupta, A. et al.
- **摘要**: 比较了LDHAL6A与其他LDH家族成员的酶动力学特性，发现其在低氧条件下活性显著增强，提示其可能通过调控乳酸稳态参与细胞缺氧应激响应。
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**注意**：以上为模拟文献，真实文献需通过PubMed、Web of Science等平台以关键词“LDHAL6A”、“recombinant protein”、“lactate dehydrogenase”检索确认。若研究较新或较冷门，建议扩大关键词或联系领域专家获取资源。
Lactate dehydrogenase A-like 6A (LDHAL6A), also known as testis-specific lactate dehydrogenase C (LDHC), is a member of the lactate dehydrogenase (LDH) family, which catalyzes the interconversion of pyruvate and lactate during glycolysis. Unlike its ubiquitously expressed homolog LDH-A (LDHA), LDHAL6A exhibits tissue-specific expression, primarily in the testes, with trace levels detected in other tissues. This isoform plays a critical role in spermatogenesis by supporting energy metabolism in sperm cells, particularly under low-oxygen conditions. Its unique substrate specificity and kinetic properties enable efficient lactate production in germ cells, maintaining redox balance and ATP generation during sperm maturation and motility.
Recombinant human LDHAL6A protein is engineered through heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Researchers utilize it to investigate metabolic adaptations in germ cells, infertility mechanisms, and isoform-specific regulatory pathways. Structural studies of recombinant LDHAL6A have revealed subtle differences in active-site architecture compared to other LDH isoforms, explaining its preferential binding to certain substrates. Moreover, it serves as a potential biomarker for male reproductive disorders and a target for contraceptive drug development. The production of recombinant LDHAL6A facilitates high-throughput screening of inhibitors and therapeutic candidates, advancing reproductive health research.
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