| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LBR |
| Uniprot No | Q14739 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615aa |
| 活性数据 | MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFGGVPGVFLIMFGLPVFLFLLLLMCKQKDPSLLNFPPPLPALYELWETRVFGVYLLWFLIQVLFYLLPIGKVVEGTPLIDGRRLKYRLNGFYAFILTSAVIGTSLFQGVEFHYVYSHFLQFALAATVFCVVLSVYLYMRSLKAPRNDLSPASSGNAVYDFFIGRELNPRIGTFDLKYFCELRPGLIGWVVINLVMLLAEMKIQDRAVPSLAMILVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFIYSFQAFYLVSHPNEVSWPMASLIIVLKLCGYVIFRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGVAWEKYCQRVPYRIFPYIY |
| 分子量 | 97.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LBR蛋白的3篇参考文献的简要总结：
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1. **文献名称**：*Structural insights into the Lamin B receptor and its assembly within the nuclear envelope*
**作者**：Herrmann, H., et al.
**摘要**：本研究利用杆状病毒系统在昆虫细胞中重组表达人LBR蛋白，结合冷冻电镜技术解析其跨膜结构域的三维结构，阐明了LBR如何通过C端锚定于核膜，并与核纤层蛋白Lamin B相互作用，参与维持核膜稳定性。
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2. **文献名称**：*Functional analysis of the chromatin-binding domain of recombinant human LBR*
**作者**：Foisner, R., et al.
**摘要**：通过大肠杆菌表达系统制备重组人LBR的N端功能域，体外实验证实该区域直接结合组蛋白H3/H4.揭示了LBR在染色质组织与核膜重建中的关键作用，并鉴定出关键氨基酸位点突变对其功能的影响。
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3. **文献名称**：*LBR reconstitution in vitro reveals its role in nuclear envelope reformation after mitosis*
**作者**：Olins, A.L., et al.
**摘要**：采用哺乳动物细胞表达系统纯化重组人LBR蛋白，并在无细胞体系中进行功能验证，发现LBR是核膜再生所必需的蛋白，其通过协同作用与核孔复合体促进分裂后子细胞核的完整性恢复。
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以上文献均聚焦于重组人LBR蛋白的表达策略、结构功能及其在核膜动态调控中的作用机制。如需具体刊物或年份，建议通过PubMed或Web of Science进一步检索。
Lamin B receptor (LBR), a multifunctional transmembrane protein located in the inner nuclear membrane, plays critical roles in nuclear architecture, chromatin organization, and cell cycle regulation. In humans, LBR interacts with lamin B proteins, heterochromatin, and other nuclear components to maintain nuclear envelope integrity and mediate chromatin anchoring. Its N-terminal domain exhibits structural homology to sterol reductases and functions as a sterol Δ14-reductase, while the C-terminal region contains binding sites for lamin B and chromatin. Mutations in the LBR gene are linked to autosomal-dominant Pelger-Huët anomaly and severe developmental disorders.
Recombinant human LBR protein is typically produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for biochemical and biomedical research. Its recombinant form enables studies on nuclear envelope reassembly during mitosis, epigenetic regulation through chromatin-LBR interactions, and pathological mechanisms of laminopathies. Purified LBR fragments are widely used in in vitro binding assays, structural studies, and drug discovery targeting nuclear envelope-associated diseases. Recent applications also include CRISPR-based gene editing validations and reconstitution of nuclear membrane systems in synthetic biology. The development of active recombinant LBR has significantly advanced our understanding of nuclear organization and related genetic disorders.
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