| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LARP2 |
| Uniprot No | Q659C4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 活性数据 | MDSRDRGPGTSSVSTSNASPSEGAPLAGSYGCTPHSFPKFQHPSHELLKENGFTQQVYHKYRRRCLSERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFRQLAWEDAKENYRYGLECLFRFYSYGLEKKFRREIFQDFQEETKKDYESGQLYGLEKFWAYLKYSQSKTQSIDPKLQEYLCSFKRLEDFRVDEADPIE |
| 分子量 | 40.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人LARP2蛋白的模拟参考文献示例(注：内容为根据相关领域研究方向的合理推演，非真实文献)：
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1. **文献名称**：*Structural Insights into Human LARP2 Protein and Its RNA Recognition Mechanism*
**作者**：Chen, Y. et al.
**摘要**：本研究通过X射线晶体学解析了重组人LARP2蛋白的RNA结合结构域三维结构，揭示了其通过保守的La模块和相邻结构域协同结合poly(A) RNA的分子机制，为理解LARP2在mRNA稳定性调控中的作用提供结构基础。
2. **文献名称**：*Functional Characterization of Recombinant LARP2 in TGF-β Signaling Pathway*
**作者**：Smith, R.J. & Kumar, P.
**摘要**：文章报道了在HEK293细胞中重组表达的LARP2蛋白可特异性结合TGF-β mRNA的3'UTR区域，并通过抑制miRNA介导的降解增强靶标mRNA的稳定性，证明其在转化生长因子信号通路中的关键调控功能。
3. **文献名称**：*Optimization of Recombinant LARP2 Production in E. coli for Biochemical Studies*
**作者**：Zhang, L. et al.
**摘要**：本研究系统优化了人源LARP2蛋白在大肠杆菌中的可溶性表达条件，开发出一种高效层析纯化策略，并验证了重组蛋白的体外RNA结合活性及热稳定性，为其后续功能研究提供可靠工具。
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**说明**：LARP2(La-related protein 2)研究多集中于RNA代谢调控，实际文献可能需查询数据库(如PubMed)。若需真实文献，建议结合关键词"LARP2 recombinant"或"LARP2 RNA binding"检索。
La-related protein 2 (LARP2) is a member of the LARP superfamily, which is evolutionarily conserved and characterized by the presence of La motif (LaM) and RNA recognition motif (RRM)-like domains. Human LARP2 plays a critical role in post-transcriptional gene regulation, particularly in RNA stability, translation, and decay processes. It binds to poly(A)-binding protein (PABP) and interacts with cytoplasmic polyadenylation elements (CPEs) in mRNA 3' untranslated regions (UTRs), linking it to mechanisms of mRNA metabolism and translational control during cellular stress, differentiation, or developmental transitions.
Recombinant human LARP2 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and functional roles. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it enables biochemical assays, structural analyses (e.g., crystallography), and investigations into its involvement in diseases such as cancer or neurodegeneration, where dysregulated mRNA translation is implicated. Its recombinant form also serves as a tool for screening potential therapeutic compounds targeting RNA-protein interactions. Studies using recombinant LARP2 have shed light on its regulatory crosstalk with other RNA-binding proteins and its context-dependent roles in cellular homeostasis, making it a focal point for understanding post-transcriptional control networks.
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