| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAPTM5 |
| Uniprot No | Q13571 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-262aa |
| 活性数据 | MDPRLSTVRQTCCCFNVRIATTALAIYHVIMSVLLFIEHSVEVAHGKASCKLSQMGYLRIADLISSFLLITMLFIISLSLLIGVVKNREKYLLPFLSLQIMDYLLCLLTLLGSYIELPAYLKLASRSRASSSKFPLMTLQLLDFCLSILTLCSSYMEVPTYLNFKSMNHMNYLPSQEDMPHNQFIKMMIIFSIAFITVLIFKVYMFKCVWRCYRLIKCMNSVEEKRNSKMLQKVVLPSYEEALSLPSKTPEGGPAPPPYSEV |
| 分子量 | 56.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人LAPTM5蛋白的虚拟参考文献(基于领域研究方向的合理推测)：
1. **标题**：*LAPTM5 regulates lysosomal function via mTOR signaling in human macrophages*
**作者**：Zhang Y, et al.
**摘要**：研究利用重组人LAPTM5蛋白，揭示其在巨噬细胞溶酶体成熟和自噬中的作用，发现LAPTM5通过与mTOR复合物互作调控代谢通路，为免疫调节提供新机制。
2. **标题**：*Expression and purification of recombinant human LAPTM5 for structural analysis*
**作者**：Lee S, et al.
**摘要**：本文建立了一种基于哺乳动物表达系统的高效重组LAPTM5蛋白纯化方法，通过X射线晶体学解析其跨膜结构域，阐明其溶酶体定位的关键氨基酸位点。
3. **标题**：*LAPTM5 promotes cancer cell invasion by modulating EGFR trafficking*
**作者**：Chen H, et al.
**摘要**：研究通过体外重组LAPTM5蛋白实验，证明其通过调控EGFR内吞和溶酶体降解促进肿瘤细胞侵袭，为靶向LAPTM5-EGFR轴的癌症治疗提供依据。
4. **标题**：*LAPTM5 deficiency disrupts B-cell receptor signaling via dysregulated protein degradation*
**作者**：Wang X, et al.
**摘要**：利用重组LAPTM5蛋白及基因敲除模型，揭示其在B细胞受体信号中的功能，证明LAPTM5通过泛素化途径调控关键信号蛋白稳定性，影响免疫应答。
(注：以上文献为示例性内容，实际研究需参考真实数据库如PubMed、Web of Science等。)
Lysosome-associated protein transmembrane 5 (LAPTM5) is a member of the LAPTM family, characterized by their lysosomal localization and multiple transmembrane domains. This ubiquitously expressed protein is particularly abundant in immune cells, including lymphocytes and macrophages. Structurally, LAPTM5 contains an N-terminal proline-rich region and five transmembrane helices, which facilitate its integration into lysosomal and endosomal membranes. Its molecular weight ranges between 35-45 kDa depending on post-translational modifications.
Functionally, LAPTM5 regulates intracellular trafficking processes by interacting with ubiquitinated proteins and modulating their lysosomal degradation. It plays critical roles in immune response regulation through its involvement in cytokine receptor signaling and antigen presentation pathways. Studies highlight its participation in T-cell development, B-cell receptor internalization, and macrophage activation. Additionally, LAPTM5 exhibits tumor suppressor properties in certain cancers by promoting apoptosis and inhibiting proliferation, though context-dependent oncogenic effects have also been reported.
Recombinant human LAPTM5 protein is typically produced in mammalian expression systems to preserve post-translational modifications and functional integrity. This engineered tool enables researchers to investigate protein-protein interactions, lysosomal trafficking mechanisms, and cellular signaling pathways in vitro. Its applications span immunotherapy development, cancer biology studies, and research on lysosomal storage disorders. Current challenges in working with recombinant LAPTM5 include maintaining its transmembrane structure in solution and achieving proper membrane integration in experimental systems.
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