| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | L3MBTL |
| Uniprot No | Q9Y468 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-772aa |
| 活性数据 | MRRREGHGTDSEMGQGPVRESQSSDPPALQFRISEYKPLNMAGVEQPPTPELRQEGVTEYEDGGAPAGDGEAGPQQAEDHPQNPPEDPNQDPPEDDSTCQCQACGPHQAAGPDLGSSNDGCPQLFQERSVIVENSSGSTSASELLKPMKKRKRREYQSPSEEESEPEAMEKQEEGKDPEGQPTASTPESEEWSSSQPATGEKKECWSWESYLEEQKAITAPVSLFQDSQAVTHNKNGFKLGMKLEGIDPQHPSMYFILTVAEVCGYRLRLHFDGYSECHDFWVNANSPDIHPAGWFEKTGHKLQPPKGYKEEEFSWSQYLRSTRAQAAPKHLFVSQSHSPPPLGFQVGMKLEAVDRMNPSLVCVASVTDVVDSRFLVHFDNWDDTYDYWCDPSSPYIHPVGWCQKQGKPLTPPQDYPDPDNFCWEKYLEETGASAVPTWAFKVRPPHSFLVNMKLEAVDRRNPALIRVASVEDVEDHRIKIHFDGWSHGYDFWIDADHPDIHPAGWCSKTGHPLQPPLGPREPSSASPGGCPPLSYRSLPHTRTSKYSFHHRKCPTPGCDGSGHVTGKFTAHHCLSGCPLAERNQSRLKAELSDSEASARKKNLSGFSPRKKPRHHGRIGRPPKYRKIPQEDFQTLTPDVVHQSLFMSALSAHPDRSLSVCWEQHCKLLPGVAGISASTVAKWTIDEVFGFVQTLTGCEDQARLFKDEARIVRVTHVSGKTLVWTVAQLGDLVCSDHLQEGKGILETGVHSLLCSLPTHLLAKLSFASDSQY |
| 分子量 | 110.66 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人L3MBTL蛋白的3篇代表性文献，信息简明整理如下：
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1. **标题**：*Structural basis for specific recognition of methylated histone H3K9 by the MBT repeats of L3MBTL1*
**作者**：Li H, Fischle W, Wang W, et al.
**摘要**：该研究解析了L3MBTL1蛋白MBT结构域结合甲基化组蛋白H3K9的分子机制，发现其通过多个MBT重复单元协同识别甲基化修饰，揭示其在染色质压缩和基因沉默中的作用。
2. **标题**：*L3MBTL1 regulates polycomb-mediated gene silencing through compaction of chromatin*
**作者**：Trojer P, Cao AR, Gao Z, et al.
**摘要**：研究证明重组L3MBTL1蛋白通过物理压缩染色质结构增强Polycomb复合体的基因沉默功能，表明其在表观遗传调控中通过染色质构象变化抑制靶基因表达。
3. **标题**：*L3MBTL1 interacts with the RNF2 component of the polycomb repressive complex and is required for its oncogenic activity*
**作者**：Qin J, Van Buren D, Huang HS, et al.
**摘要**：发现L3MBTL1与Polycomb复合体亚基RNF2相互作用，并通过调节靶基因(如抑癌基因)的甲基化依赖性抑制，驱动白血病的发生，提示其作为潜在治疗靶点。
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以上文献均涉及L3MBTL蛋白的结构、表观遗传调控功能及疾病相关性，可通过PubMed或期刊官网(如*Nature*、*Molecular Cell*)进一步查阅。
Recombinant human L3MBTL protein is a genetically engineered form of the L3MBTL (Lethal(3)malignant brain tumor-like) protein, a member of the MBT (malignant brain tumor) domain-containing protein family. Endogenously, L3MBTL plays critical roles in epigenetic regulation, chromatin remodeling, and transcriptional repression. It recognizes mono- and dimethylated lysine residues on histones (e.g., H4K20me1/2), linking histone modification states to downstream cellular processes such as DNA repair, cell cycle control, and tumor suppression. Dysregulation of L3MBTL has been implicated in malignancies, developmental disorders, and other diseases linked to chromatin instability.
The recombinant protein, typically expressed in systems like *E. coli* or mammalian cells, retains the functional MBT domains required for binding methylated histones and interacting with partner proteins like Polycomb group proteins. Its production enables detailed biochemical and structural studies, facilitating the exploration of chromatin-associated mechanisms. Researchers utilize recombinant L3MBTL to dissect its role in repressing oncogenic pathways, its crosstalk with DNA damage response systems, and its potential as a therapeutic target. Additionally, it serves as a tool to screen small molecules that modulate methyl-lysine binding activity, offering avenues for drug development in cancers driven by epigenetic dysregulation. Studies also highlight its involvement in maintaining genomic integrity, underscoring its relevance in aging and degenerative diseases.
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