| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | L3MBTL4 |
| Uniprot No | Q8NA19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 活性数据 | MKQPNRKRKL NMDSKERLDQ DGRLEQAEEE KKPKDSTTPL SHVPSAAAQG AWSWEWYLKE QKAVAAPVEL FSKDQSFPEH ENGFQIGMRL EGIDPRHPSV FCVLSVAEVC GYRLRLHFDG YLSCYDFWTN AGSPDIHPVG WCEKTKHELH IPKGYRKDKF VWMDYLKACK LQNAPKKLFR NRSPNGPMSK EFQVGMKLEA VDRKNPSLVC VATIADIVED RLLVHFDNWD DSYDYWCDVN SPYVQPVGWC QENGRTLIAP QGYPNPENFS WTEYLEATQT NAVPAKVFKM RLPHGFLPNM KLEVVDKRNP RLIRVATIVD VDDQRVKVHF DGWDHKYDYW VEADSPDIHP IGWCDVTGHP LEVPQRTNDL KILPGQAVCP TPGCRGIGHI RGPRYSGHHS AFGCPYSDMN LKKEATLHDR LREQTQANLE SDSSHSKSKS LCSLNFNGKH EKVNSQPRLV QQAKCLKIKG KEDIDLDNLF RVLVLHPRGL EYSVEQAQQV LHQSVSMSTV SAHPFRDLPL GREQHCKLLP GVADIRASQV ARWTVDEVAE FVQSLLGCEE HAKCFKKEQI DGKAFLLLTQ TDIVKVMKIK LGPALKIYNS ILMFRHSQEL PEEDIASGQE VRG |
| 分子量 | 71.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人L3MBTL4蛋白的三篇参考文献及其摘要概要：
---
### 1. **"Structural insights into L3MBTL4 recognition of monomethylated histones"**
**作者**: Smith J, et al.
**摘要**: 本研究解析了重组人L3MBTL4蛋白的MBT结构域与单甲基化组蛋白H4K20结合的晶体结构，揭示了其通过疏水口袋选择性识别单甲基化赖氨酸的分子机制，为理解其在染色质压缩和基因沉默中的作用提供结构基础。
---
### 2. **"L3MBTL4 regulates DNA repair through interaction with 53BP1 in the p53 pathway"**
**作者**: Li X, Wang Y, et al.
**摘要**: 文章发现重组人L3MBTL4蛋白与DNA损伤修复蛋白53BP1直接结合，通过调控p53信号通路影响双链断裂修复效率，其缺失导致基因组不稳定性和肿瘤易感性增加，提示L3MBTL4在癌症发生中的潜在作用。
---
### 3. **"Recombinant L3MBTL4 facilitates the assembly of a polycomb-repressive complex in vitro"**
**作者**: Tanaka M, et al.
**摘要**: 研究报道了重组人L3MBTL4蛋白在体外作为支架蛋白参与多梳抑制复合体(PRC1)的组装，通过促进RING1B与E2泛素结合酶的相互作用，增强H2A的泛素化活性，阐明了其在表观遗传调控中的功能机制。
---
*注：以上文献为示例性内容，实际研究请以具体发表的论文为准。建议通过PubMed或Web of Science以“L3MBTL4”、“recombinant protein”、“epigenetics”等关键词检索最新文献。*
**Background of Recombinant Human L3MBTL4 Protein**
The L3MBTL4 (lethal(3)malignant brain tumor-like 4) protein is a chromatin-associated factor belonging to the Polycomb group (PcG) protein family, known for its role in epigenetic regulation. It contains multiple malignant brain tumor (MBT) domains, which enable binding to methylated lysine residues on histones, particularly H4K20me1 and H3K36me3. facilitating chromatin compaction and transcriptional repression. Structurally, L3MBTL4 acts as a bridging molecule, recruiting chromatin-modifying complexes to maintain genomic stability and regulate gene expression.
Recombinant human L3MBTL4 is engineered using expression systems like *E. coli* or mammalian cells, enabling precise study of its biochemical and functional properties *in vitro*. This protein is pivotal in investigating its role in chromatin remodeling, DNA repair, and cell cycle regulation. Dysregulation of L3MBTL4 has been linked to cancers, developmental disorders, and neurodegenerative diseases, highlighting its therapeutic potential. For instance, its interaction with the tumor suppressor p53 and involvement in repressing oncogenic pathways underscore its relevance in oncology research.
Studies utilizing recombinant L3MBTL4 have advanced understanding of how Polycomb proteins orchestrate epigenetic silencing and maintain cellular identity. Additionally, it serves as a tool for screening inhibitors targeting MBT domain-mediated interactions, paving the way for novel epigenetic therapies. Overall, recombinant L3MBTL4 is a critical reagent for dissecting chromatin biology and exploring therapeutic interventions in diseases linked to epigenetic dysfunction.
×
