| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP13-3 |
| Uniprot No | Q3SY46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 活性数据 | MSYNCCSRNFSSCSHGGYLHYPGSSCGSSYPSNLVYSTDLCSPSTCQLGSSLYRGCQETCWRPNSCQTLCVESSPCHTSCYYPRTHMLCNSCLTMHVGSRGFGSNSCCSLSCGSRSCSSLGCGSNGFRYLNYRIHTSPSQSYRSRFCHPIYFPPRRWFHSSCYQPFCRSGFY |
| 分子量 | 45.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP13-3蛋白的3篇参考文献示例(注：以下文献为模拟生成，非真实存在)：
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1. **《重组人KRTAP13-3蛋白的表达及其对毛发纤维力学性能的影响》**
*作者：Zhang L, et al.*
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人KRTAP13-3蛋白，通过体外模拟实验发现该蛋白显著增强了人工毛纤维的弹性和抗拉强度，提示其在毛囊角蛋白网络中的结构支持作用。
2. **《KRTAP13-3基因多态性与雄激素性脱发的关联研究》**
*作者：Wang Y, et al.*
**摘要**：通过对脱发患者群体的基因测序，发现KRTAP13-3的特定单核苷酸多态性(SNP)与疾病风险显著相关，重组蛋白功能实验进一步表明其突变体会削弱毛干中角蛋白的交联效率。
3. **《基于CRISPR/Cas9的KRTAP13-3敲除模型揭示其在表皮屏障中的作用》**
*作者：Chen H, et al.*
**摘要**：构建KRTAP13-3敲除小鼠模型，发现表皮屏障功能受损且毛发易断裂。补充重组人KRTAP13-3蛋白后表型部分恢复，证实其在维持皮肤和毛发完整性中的关键作用。
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以上内容综合了蛋白重组技术、疾病关联及功能机制研究，符合该领域常见研究方向。实际文献需通过学术数据库(如PubMed、Google Scholar)检索核实。
Keratatin-associated protein 13-3 (KRTAP13-3) is a member of the keratin-associated protein (KAP) family, which plays a critical role in the structural organization and mechanical integrity of hair and wool fibers. These proteins are encoded by genes clustered in the human chromosome 21q22.1 region and are categorized into high sulfur, ultra-high sulfur, or high glycine-tyrosine subfamilies based on amino acid composition. KRTAP13-3. a high sulfur KAP, is expressed in the hair follicle cortex and contributes to cross-linking keratin intermediate filaments through disulfide bonds, influencing hair texture, elasticity, and resistance to environmental stress. The recombinant human KRTAP13-3 protein is artificially synthesized using expression systems like *E. coli* or mammalian cells, enabling scalable production for functional studies. Its recombinant form typically includes tags (e.g., His-tag) for purification and detection. Research on KRTAP13-3 focuses on understanding its role in hair disorders, genetic variations linked to trichological pathologies, and potential applications in biomaterial engineering. Studies also explore its interactions with keratins and other KAPs to decipher hair formation mechanisms. Despite its importance, challenges remain in resolving its precise 3D structure and post-translational modifications. Investigating recombinant KRTAP13-3 may advance diagnostics for hair-related diseases or inspire bio-inspired materials mimicking hair’s resilience.
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