| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP11-1 |
| Uniprot No | Q8IUC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-163aa |
| 活性数据 | MSFNCSTRNCSSRPIGGRCIVPVAQVTTTSTTDADCLGGICLPSSFQTGSWLLDHCQETCCEPTACQPTCYRRTSCVSNPCQVTCSRQTTCISNPCSTTYSRPLTFVSSGCQPLGGISSVCQPVGGISTVCQPVGGVSTVCQPACGVSRTYQQSCVSSCRRTC |
| 分子量 | 18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP11-1蛋白的参考文献示例：
1. **"Expression and Characterization of Recombinant Human KRTAP11-1 in Escherichia coli"**
*Author: Zhang L, et al.*
该研究成功构建了KRTAP11-1的原核表达系统，优化蛋白纯化条件，证实重组蛋白的稳定性及与角蛋白相互作用的体外功能。
2. **"Structural Insights into Human KRTAP11-1: Implications for Hair Follicle Development"**
*Author: Gupta S, et al.*
通过晶体学解析KRTAP11-1的三维结构，揭示其C端结构域在毛发角蛋白网络组装中的关键作用，为遗传性毛发疾病研究提供依据。
3. **"KRTAP11-1 Modulates Epidermal Differentiation via Wnt/β-catenin Signaling"**
*Author: Wang Y, et al.*
功能研究发现重组KRTAP11-1通过激活Wnt通路促进表皮细胞分化，提示其在皮肤屏障修复中的潜在应用价值。
4. **"Aberrant Expression of KRTAP11-1 in Cutaneous Squamous Cell Carcinoma"**
*Author: Kim HJ, et al.*
免疫组化分析显示KRTAP11-1在皮肤鳞癌中异常高表达，体外实验证实其过表达促进肿瘤细胞迁移，提示其作为癌症标志物的可能性。
(注：以上文献为示例性虚构内容，实际研究需查询具体数据库。)
Keratin-associated protein 11-1 (KRTAP11-1) is a member of the keratin-associated protein (KAP) family, which plays critical roles in the structural organization and mechanical properties of hair and skin. These proteins interact with keratin intermediate filaments to form a robust cytoskeletal network, contributing to the integrity and elasticity of epithelial tissues. KRTAP11-1 is encoded by the *KRTAP11-1* gene, located on human chromosome 21q22.11. and is characterized by its high sulfur content and cysteine-rich sequences, typical of KAPs involved in disulfide bond-mediated crosslinking during hair shaft formation. Though its precise biological function remains less explored compared to other KAPs, studies suggest it participates in hair matrix assembly, influencing hair texture and stability. Dysregulation of KAPs, including KRTAP11-1. has been tentatively linked to hair disorders, though direct evidence is limited. Recombinant human KRTAP11-1 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling investigations into its biochemical interactions, structural contributions to keratin networks, and potential therapeutic applications. Its recombinant form aids in deciphering molecular mechanisms underlying hair biology and related genetic diseases. However, challenges in protein solubility, purification, and post-translational modifications reflect broader hurdles in studying cysteine-rich KAPs. Current research focuses on characterizing its role in epithelial resilience and exploring its utility in biomaterials or targeted therapies for keratin-based pathologies.
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