| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP10-6 |
| Uniprot No | P60371 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-365aa |
| 活性数据 | MAASTMSVCSSDLSYGSRVCLPGSCDSCSDSWQVDDCPESCCEPPCCAPAPCLSLVCTPVSRVSSPCCPVTCEPSPCQSGCTSSCTPSCCQQSSCQLACCASSPCQQACCVPVCCKTVCCKPVCCVSVCCGDSSCCQQSSCQSACCTSSPCQQACCVPVCCKPVCSGISSSCCQQSSCVSCVSSPCCQAVCEPSPCQSGCTSSCTPSCCQQSSCQPTCCTSSPCQQACCVPVCCVPVCCVPTCSEDSSSCCQQSSCQPACCTSSPCQHACCVPVCSGASTSCCQQSSCQPACCTASCCRPSSSVSLLCHPVCKSTCCVPVPSCGASASSCQPSCCRTASCVSLLCRPMCSRPACYSLCSGQKSSC |
| 分子量 | 40.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP10-6蛋白的参考文献示例(注：部分信息为示例性内容，实际文献可能需通过学术数据库验证):
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1. **文献名称**: "Expression and Characterization of Recombinant Human KRTAP10-6 Protein in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功克隆并纯化了重组人KRTAP10-6蛋白，通过质谱和圆二色谱验证其正确折叠。该研究为后续功能研究提供了高纯度蛋白来源。
2. **文献名称**: "KRTAP10-6 Interactions with Hair Keratins: Insights from Recombinant Protein Binding Assays"
**作者**: Zhang L, et al.
**摘要**: 通过重组KRTAP10-6蛋白与毛发角蛋白的体外结合实验，揭示其在毛发纤维交联中的潜在作用，并发现其表达水平与毛发强度相关。
3. **文献名称**: "Genetic Variation in KRTAP10-6 and Its Association with Alopecia Areata"
**作者**: Tanaka Y, et al.
**摘要**: 通过重组KRTAP10-6蛋白的功能分析，发现该蛋白的某些突变体与斑秃患者的毛囊异常相关，提示其在毛发疾病中的分子机制。
4. **文献名称**: "Structural Insights into KRTAP10-6 via X-ray Crystallography"
**作者**: Lee S, et al.
**摘要**: 报道了重组KRTAP10-6蛋白的晶体结构，揭示其特有的二硫键网络和与其他角蛋白结合的结构域，为设计靶向分子奠定基础。
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**注意**: 上述文献为示例，实际研究中KRTAP10-6相关论文可能较少。建议通过PubMed、Web of Science等平台使用关键词“KRTAP10-6 recombinant”或“keratin-associated protein 10-6”检索最新文献。
Recombinant human KRTAP10-6 protein is a engineered variant of the keratin-associated protein 10-6 (KRTAP10-6), a member of the keratin-associated protein (KAP) family. These proteins play critical roles in hair and nail formation by interacting with keratin intermediate filaments to form rigid structures, contributing to mechanical strength, flexibility, and chemical resistance. KRTAP10-6. encoded by the *KRTAP10-6* gene on human chromosome 21. is a cysteine-rich protein predominantly expressed in hair follicles. It facilitates disulfide bond-mediated cross-linking, stabilizing the hair shaft matrix.
Interest in recombinant KRTAP10-6 arises from its potential applications in biomedical and cosmetic research. Recombinant production, typically via bacterial or mammalian expression systems, enables scalable synthesis for functional studies. Researchers aim to elucidate its precise role in hair biology, particularly its interactions with keratins and impact on hair disorders like alopecia or structural abnormalities. Additionally, its properties may inspire biomaterials for tissue engineering or novel hair-care formulations.
Current studies focus on characterizing its structural motifs, post-translational modifications, and cross-linking efficiency. Challenges include maintaining solubility and native conformation during purification. Understanding KRTAP10-6 could advance therapies for keratin-related diseases and enhance biotechnological applications leveraging its unique physicochemical traits.
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