| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP10-2 |
| Uniprot No | P60368 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 活性数据 | MAASTMSICSSACTNSWQVDDCPESCCELPCGTPSCCAPAPCLTLVCTPVSCVSSPCCQAACEPSACQSGCTSSCTPSCCQQSSCQPACCTSSPCQQACCVPVCCKPVCCVPVCCGASSCCQQSSCQPACCASSSCQQSCRVPVCCKAVCCVPTCSESSSSCCQQSSCQPACCTSSPCQQSCCVSVCCKPVCCKSICCVPVCSGASSPCCQQSSCQPACCTSSCCRPSSSVSLLCRPVCSRPASCSFSSGQKSSC |
| 分子量 | 55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP10-2蛋白的3条参考文献示例：
1. **"Cloning and Expression of Human KRTAP10-2 in Escherichia coli: A Structural Insight into Hair Keratin-Associated Proteins"**
*Zhang, L., Wang, Y., & Li, H.*
该研究成功克隆了KRTAP10-2基因，并在大肠杆菌中高效表达重组蛋白。通过质谱和Western blot验证了蛋白的分子量及特异性，为后续功能研究提供了纯化蛋白样本。研究表明该蛋白具有典型角蛋白结构域，可能在毛发力学性能中发挥作用。
2. **"Structural Characterization of Recombinant KRTAP10-2 by X-ray Crystallography Reveals Disulfide Bond-mediated Stability"**
*Smith, J.P., Tanaka, K., & Gupta, R.*
通过X射线晶体学解析了KRTAP10-2的3D结构，揭示了其核心α-螺旋区及二硫键网络对稳定性的贡献。结构分析表明，该蛋白可能通过与角蛋白纤维交联影响毛发强度，为遗传性毛发疾病的突变位点研究提供了依据。
3. **"Functional Analysis of KRTAP10-2 in Epidermal Differentiation Using CRISPR-Cas9 Keratinocyte Models"**
*Chen, X., Liu, F., & Zhou, M.*
利用CRISPR-Cas9技术构建人角质细胞敲除模型，发现KRTAP10-2缺失导致角化包膜形成异常。研究证实该蛋白通过调控脂质代谢相关基因(如ABCA12)参与皮肤屏障功能，提示其与鱼鳞病等角化障碍疾病的潜在关联。
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注：以上文献为模拟示例，实际研究中请通过学术数据库检索最新成果。
**Background of Recombinant Human KRTAP10-2 Protein**
KRTAP10-2 (Keratin-Associated Protein 10-2) belongs to the keratin-associated protein (KAP) family, which plays a critical role in forming and stabilizing hair shaft structures by interacting with keratin intermediate filaments. These proteins are essential for hair strength, elasticity, and structural integrity. The KRTAP10-2 gene, located on human chromosome 21q22.1. encodes a cysteine-rich protein predominantly expressed in the hair cortex.
Recombinant KRTAP10-2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions, and functions. Its production enables detailed exploration of molecular mechanisms underlying hair formation, keratin network assembly, and potential contributions to hair disorders like hypotrichosis or hair fragility. Additionally, recombinant KRTAP10-2 serves as a tool for structural analysis (e.g., X-ray crystallography) or screening compounds targeting hair-related pathologies. Research on this protein may advance cosmetic science, dermatological therapies, and understanding genetic hair abnormalities linked to KRTAP mutations.
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