| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT83 |
| Uniprot No | P78385 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 活性数据 | MTCGFNSIGCGFRPGNFSCVSACGPRPSRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFAGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRILQSHISDTSVVVKLDNSRDLNMDCIVAEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVEAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNLVVSTGLCKPCGQLNTTCGGGSCGQGRH |
| 分子量 | 80.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人KRT83蛋白相关的虚构参考文献示例(因真实文献可能存在限制，建议通过学术数据库核实)：
1. **"Functional Characterization of Recombinant Human Keratin 83 in Hair Follicle Biology"**
*Authors: Smith J, et al.*
**摘要**：通过大肠杆菌表达系统成功制备重组人KRT83蛋白，证实其可自组装成中间丝结构，并在体外模型中增强毛囊角质细胞的机械稳定性，暗示其在毛发疾病中的潜在作用。
2. **"Expression and Purification of Recombinant KRT83 for Structural Analysis"**
*Authors: Lee H, et al.*
**摘要**：优化了哺乳动物细胞表达的重组KRT83纯化流程，结合X射线晶体学解析其N端结构域，揭示了与KRT84异源二聚化的分子机制，为遗传性皮肤疾病突变研究提供基础。
3. **"Role of Recombinant KRT83 in Epidermal Barrier Dysfunction Models"**
*Authors: Garcia R, et al.*
**摘要**：研究显示重组KRT83蛋白可修复角质细胞分化模型中的屏障功能缺陷，并证明其与LORICRIN蛋白的相互作用，为开发针对鱼鳞病的生物制剂提供依据。
**注意**：以上内容为模拟格式，具体研究需查阅真实文献(可尝试在PubMed、Google Scholar搜索关键词：recombinant KRT83、keratin 83 expression)。
Recombinant human KRT83 protein is a genetically engineered form of the keratin 83 (KRT83) protein, a member of the type II hair keratin family. KRT83. also known as K6HF, is encoded by the *KRT83* gene located on chromosome 12q13.13 in humans. This protein is primarily expressed in the hair cortex and plays a critical role in the structural integrity and mechanical strength of hair fibers. It polymerizes with type I keratins to form intermediate filaments, which contribute to the resilience and elasticity of hair.
Mutations in *KRT83* have been linked to hereditary hair disorders, such as monilethrix and hypotrichosis, underscoring its biological significance. Recombinant KRT83 is typically produced in bacterial or mammalian expression systems, enabling researchers to study its biochemical properties, interactions, and pathological mechanisms in vitro.
This recombinant protein serves as a vital tool in dermatological research, particularly in understanding hair follicle biology, keratin-associated diseases, and developing therapeutic strategies. Applications include antibody production, structural analysis, and investigating the molecular basis of keratinopathies. Its availability in a purified, bioactive form accelerates studies on hair disorders and potential regenerative therapies.
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