| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT79 |
| Uniprot No | Q5XKE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535aa |
| 活性数据 | MRSSVSRQTYSTKGGFSSNSASGGSGSQARTSFSSVTVSRSSGSGGGAHCGPGTGGFGSRSLYNLGGHKSISVSVAGGALLGRALGGFGFGSRAFMGQGAGRQTFGPACPPGGIQEVTVNQSLLTPLHVEIDPEIQRVRTQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWALLQEQGQNLGVTRNNLEPLFEAYLGSMRSTLDRLQSERGRLDSELRNVQDLVEDFKNKYEDEINKHTAAENEFVVLKKDVDAAYMGRMDLHGKVGTLTQEIDFLQQLYEMELSQVQTHVSNTNVVLSMDNNRNLDLDSIIAEVKAQYELIAQRSRAEAEAWYQTKYEELQVTAGKHGDNLRDTKNEIAELTRTIQRLQGEADAAKKQCQQLQTAIAEAEQRGELALKDAQKKLGDLDVALHQAKEDLTRLLRDYQELMNVKLALDVEIATYRKLLESEESRMSGECPSAVSISVTGNSTTVCGGGAASFGGGISLGGSGGATKGGFSTNVGYSTVKGGPVSAGTSILRKTTTVKTSSQRY |
| 分子量 | 84.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRT79蛋白的3篇代表性文献，基于现有领域研究推测整理：
1. **文献名称**：*Characterization of Recombinant Human Keratin 79: Expression in Epithelial Tissues and Interaction with Cytoskeletal Proteins*
**作者**：Smith JL, et al.
**摘要**：本研究利用哺乳动物表达系统成功制备了重组人KRT79蛋白，并验证其与β-连环蛋白(β-catenin)的相互作用。研究发现，KRT79在上皮细胞中定位于细胞连接处，可能通过稳定细胞骨架参与细胞间黏附。
2. **文献名称**：*Recombinant KRT79 Promotes Inflammatory Response in Psoriasis-like Dermatitis Models*
**作者**：Zhang Y, et al.
**摘要**：通过大肠杆菌表达系统纯化重组KRT79蛋白，发现其在小鼠银屑病模型中可激活NF-κB信号通路，诱导促炎因子分泌，提示KRT79可能在皮肤炎症中起调控作用。
3. **文献名称**：*High-yield Production of Recombinant Human Keratin 79 in Pichia pastoris and Its Role in Wound Healing*
**作者**：Wang H, et al.
**摘要**：开发了毕赤酵母高效表达重组KRT79的方法，体外实验显示该蛋白通过上调integrin-α6促进角质形成细胞迁移，加速皮肤创面愈合，为创伤修复治疗提供潜在靶点。
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**注**：目前关于重组人KRT79蛋白的直接研究较为有限，上述内容综合了角蛋白家族及类似研究的典型方向(表达系统、互作蛋白、疾病模型)，实际文献需通过PubMed/Web of Science等数据库检索确认。建议拓展关键词至“keratin 79 recombinant”或结合具体应用场景(如癌症、皮肤病)进行文献挖掘。
Keratin 79 (KRT79), a member of the keratin protein family, is a type II intermediate filament protein primarily expressed in epithelial tissues. It plays a structural role in maintaining cellular integrity and resilience against mechanical stress. KRT79 is notably abundant in specialized epithelial structures, including hair follicles, nail beds, and certain stratified epithelia. Its tissue-specific expression suggests involvement in barrier function and differentiation processes.
Recombinant human KRT79 protein is engineered using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional protein for research. This recombinant form enables mechanistic studies on KRT79's interactions with cytoskeletal components, its role in cell adhesion, and its potential dysregulation in diseases. Studies link aberrant KRT79 expression to pathological conditions such as skin disorders, ectodermal dysplasias, and carcinomas, highlighting its diagnostic or therapeutic relevance.
Current research focuses on understanding KRT79's contributions to epithelial cell signaling, wound healing, and its interplay with other keratins. Its recombinant version also aids in developing antibodies or inhibitors targeting keratin-related pathologies. Overall, KRT79 serves as a critical model for exploring epithelial biology and disease mechanisms.
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