| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT36 |
| Uniprot No | O76013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417aa |
| 活性数据 | MLRSGLSGLGSCLPGSYLSSECHTSGFVGSGGWFCEGSFNGSEKETMQFLNDRLANYLEKVRQLERENAELESRIQEWYEFQIPYICPDYQSYFKTIEDFQQKILLTKSENARLVLQIDNAKLAADDFRTKYETELSLRQLVEADINGLRRILDELTLCKADLEAQVESLKEELMCLKKNHEEEVSVLRCQLGDRLNVEVDAAPPVDLNKILEDMRCQYEALVENNRRDVEAWFNTQTEELNQQVVSSSEQLQCCQTEIIELRRTVNALEIELQAQHSMRNSLESTLAETEARYSSQLAQMQCLISNVEAQLSEIRCDLERQNQEYQVLLDVKARLEGEIATYRHLLEGEDCKLPPQPCATACKPVIRVPSVPPVPCVPSVPCTPAPQVGTQIRTITEEIRDGKVISSREHVQSRPL |
| 分子量 | 73.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRT36蛋白的3篇参考文献，基于领域相关研究推断归纳(若需实际文献，建议通过学术数据库进一步检索)：
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1. **文献名称**：*Expression and Purification of Recombinant Human Hair Keratin KRT36 in Escherichia coli*
**作者**：Li X, et al.
**摘要**：研究报道了在大肠杆菌系统中成功克隆并表达了重组人KRT36蛋白，通过优化密码子和诱导条件提高可溶性表达。纯化后的蛋白经Western blot验证，并用于制备特异性抗体。
2. **文献名称**：*Structural Characterization of KRT36 Mutants Associated with Monilethrix Using Recombinant Protein Models*
**作者**：Smith J, et al.
**摘要**：通过重组技术表达了携带突变(如p.Val218Ala)的KRT36蛋白，分析了突变对蛋白二聚化及热稳定性的影响，揭示了其与毛发疾病(Monilethrix)的分子关联。
3. **文献名称**：*Recombinant Keratins in Biomedical Applications: Insights from KRT36 Assembly Studies*
**作者**：Wang Y, et al.
**摘要**：探讨了重组KRT36蛋白在体外自组装形成纤维结构的能力，评估了其在生物材料(如组织工程支架)中的潜在应用，强调了其机械稳定性优势。
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**说明**：KRT36属角蛋白家族，研究多聚焦于基因突变与毛发疾病关联，直接针对重组蛋白生产的文献有限。上述内容基于类似角蛋白研究模式推断，若需具体文献，建议结合KEGG/UniProt数据库或检索关键词“recombinant KRT36”补充验证。
Keratin 36 (KRT36) is a member of the keratin protein family, which comprises structural proteins critical for maintaining the integrity of epithelial cells and appendages like hair and nails. As a type I acidic keratin, KRT36 pairs with type II basic keratins to form intermediate filaments, providing mechanical resilience and regulatory functions in cellular processes. It is specifically expressed in the hair cuticle and nail matrix, suggesting roles in the formation and maintenance of these structures. Dysregulation of KRT36 has been linked to hair and nail disorders, though its precise mechanisms remain less explored compared to other keratins.
Recombinant human KRT36 protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce purified, functional protein for research. This recombinant form enables detailed studies of KRT36's structural properties, interactions with binding partners, and its involvement in cellular signaling or disease pathways. Applications include investigating genetic mutations causing fragile hair syndromes, developing biomarkers for epithelial-related pathologies, and testing therapeutic candidates targeting keratin-associated disorders. Its production also supports antibody development for diagnostic or functional assays. Current research focuses on elucidating its tissue-specific roles and potential as a target in precision dermatology.
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