| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT27 |
| Uniprot No | Q7Z3Y8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459aa |
| 活性数据 | MSVRFSSTSRRLGSCGGTGSVRLSSGGAGFGAGNTCGVPGIGSGFSCAFGGSSSAGGYGGGLGGGSASCAAFTGNEHGLLSGNEKVTMQNLNDRLASYLENVRALEEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDELKNQIISATTSNAHVVLQNDNARLTADDFRLKFENELALHQSVEADINGLRRVLDELTLCRTDLEIQLETLSEELAYLKKNHEEEMKALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFNEKSASLQQQISDDAGATTSARNELIEMKRTLQTLEIELQSLLATKHSLECSLTETESNYCAQLAQIQAQIGALEEQLHQVRTETEGQKLEYEQLLDIKVHLEKEIETYCLLIDGEDGSCSKSKGYGGPGNQTKDSSKTTIVKTVVEEIDPRGKVLSSRVHTVEEKSTKVNNKNEQRVSS |
| 分子量 | 50.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRT27蛋白的参考文献示例(注：以下信息为假设性内容，实际文献需通过学术数据库验证)：
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1. **标题**: *Structural characterization and functional analysis of recombinant human KRT27 in epithelial differentiation*
**作者**: Smith A, et al. (2020)
**摘要**: 本研究通过大肠杆菌系统表达并纯化了重组人KRT27蛋白，揭示了其与角蛋白网络相互作用的分子机制，发现其在上皮细胞分化和屏障功能中起关键作用。
2. **标题**: *Recombinant KRT27 as a potential biomarker in squamous cell carcinoma*
**作者**: Lee J, Park S (2019)
**摘要**: 报道了重组KRT27蛋白在鳞状细胞癌细胞中的异常高表达，通过免疫组化验证其作为肿瘤标志物的潜力，并提出其在癌症诊断中的临床应用前景。
3. **标题**: *Expression optimization and biochemical properties of human KRT27 recombinant protein*
**作者**: Zhang Y, et al. (2021)
**摘要**: 利用哺乳动物表达系统优化重组KRT27的生产条件，分析了其热稳定性和磷酸化修饰特性，为大规模制备及结构研究提供了技术基础。
4. **标题**: *KRT27 interactions with desmosomal proteins revealed by recombinant protein assays*
**作者**: Johnson R, et al. (2018)
**摘要**: 通过体外结合实验证明重组KRT27与桥粒蛋白DSG1的相互作用，揭示了其在皮肤细胞间粘附中的功能，并探讨了突变体对皮肤疾病的影响。
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**注意**：以上文献为示例，实际研究中需通过PubMed、Web of Science或Google Scholar等平台检索真实文献。建议使用关键词“recombinant KRT27 human”或结合具体研究领域(如“癌症”“表皮分化”)筛选。
Keratin 27 (KRT27) is a member of the keratin family, a group of structural proteins crucial for maintaining the integrity and mechanical stability of epithelial cells. As a type I acidic keratin, KRT27 pairs with specific type II basic keratins (e.g., KRT77) to form intermediate filaments that contribute to cell shape, adhesion, and stress resilience. It is constitutively expressed in stratified and cornified epithelia, including skin, hair follicles, and nail beds, where it supports barrier function and tissue repair. Mutations in *KRT27* have been linked to inherited skin disorders like epidermolysis bullosa simplex (EBS), where weakened keratin networks cause cell fragility and blistering.
Recombinant human KRT27 protein is engineered using bacterial, yeast, or mammalian expression systems to produce purified, functional protein for research and therapeutic applications. Its production enables studies on keratin filament assembly, protein-protein interactions, and cellular responses to mechanical stress. Researchers utilize recombinant KRT27 to investigate disease mechanisms, screen potential drugs targeting keratinopathies, or develop biomarkers for epithelial cancers. Additionally, it serves as a tool to study post-translational modifications (e.g., phosphorylation) that regulate keratin dynamics during wound healing or apoptosis. Despite limited characterization compared to other keratins, ongoing research on KRT27 aims to unravel its tissue-specific roles and therapeutic potential in epithelial disorders.
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