| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT222P |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 活性数据 | MDKDEEALKAAQAELKEARRQWHHLQVEIESLHAVERGLENSLHASEQHYQMQLQDLETVIEGLEKELQEVRRGIEKQLQEHEMLLNTKMRLEQEIATYRHLLEKEEIRYYGCIQGGKKDKKPTTSRVGFVLPSAIINEISFTTKVPQKYENENVETVTKQAILNGSIVKESTEAHGTIQTEKVDEVIKEWEGSFFKDNPRLRKKSVSLRFDLHLAATDEGCLETKQDNLPDIEVRLIMRRSCSIPSIKPPSTAN |
| 分子量 | 54.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于角蛋白相关研究的推测性参考文献示例(注：KRT222P目前未见明确文献报道，以下内容为假设性示例)：
1. **《Characterization of recombinant human KRT222P protein expression in E. coli》**
- **作者**: Li, X. et al.
- **摘要**: 本研究通过大肠杆菌系统成功表达重组人KRT222P蛋白，优化纯化条件并验证其结构稳定性，为后续功能研究奠定基础。
2. **《KRT222P pseudogene-encoded protein interacts with cytoskeletal components in vitro》**
- **作者**: García, R. & Müller, S.
- **摘要**: 发现重组KRT222P蛋白能与微管蛋白结合，提示其可能参与细胞骨架调控，为假基因编码蛋白的功能研究提供新方向。
3. **《Functional analysis of KRT222P in epidermal differentiation using recombinant protein models》**
- **作者**: Chen, H. et al.
- **摘要**: 利用重组KRT222P蛋白处理角质细胞，发现其抑制分化相关基因表达，暗示其在皮肤屏障中的潜在调控作用。
4. **《Bioinformatics and structural prediction of the KRT222P pseudogene-derived protein》**
- **作者**: Park, J. & Kim, T.
- **摘要**: 通过计算模型预测KRT222P蛋白的三维结构，并分析其与角蛋白家族的功能相似性，推测其可能在应激反应中发挥作用。
**说明**：目前公开数据库中未见KRT222P蛋白的详细研究，以上内容为结合角蛋白假基因研究范式的假设性描述。建议通过PubMed或UniProt确认最新进展。
Keratins are a diverse family of structural proteins essential for maintaining epithelial and hair/nail integrity. The human genome contains over 50 functional keratin genes, classified as type I (acidic) or type II (basic), which form heteropolymers to create intermediate filaments. KRT222P (Keratin 222 Pseudogene) is annotated as a transcribed pseudogene within the type I keratin cluster on chromosome 17q21.2. Pseudogenes are traditionally considered non-functional due to mutations disrupting their coding potential, but emerging evidence suggests some may retain regulatory roles or even encode truncated proteins under specific conditions.
Recombinant human KRT222P protein is engineered to study its potential biological significance. Its hypothetical structure includes conserved keratin domains (e.g., central α-helical rod), though pseudogene status implies possible sequence variations compared to functional keratins. Production typically involves cloning partial or full-length cDNA into expression systems (e.g., E. coli, mammalian cells), followed by purification via affinity tags. Research focuses on exploring its interaction with other keratins, possible roles in cellular stress responses, or aberrant expression in diseases like cancer. However, functional studies remain limited, reflecting ongoing debates about pseudogene roles in human biology. Current applications include antibody validation, protein interaction assays, and investigating evolutionary divergence of keratin genes.
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