| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KNTC2 |
| Uniprot No | O14777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-642aa |
| 活性数据 | MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE |
| 分子量 | 73.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KNTC2蛋白的3篇参考文献示例(注：内容为模拟生成，仅供参考)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human KNTC2 in Mitotic Regulation"*
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究利用哺乳动物表达系统成功制备了重组人KNTC2蛋白，并通过免疫荧光和活细胞成像技术证明其参与有丝分裂中期染色体排列。实验表明，KNTC2通过与微管结合蛋白相互作用，调控纺锤体组装及染色体分离的精确性。
2. **文献名称**: *"Structural Insights into KNTC2 Interaction with the Kinetochore Complex"*
**作者**: Gupta R, Patel S, et al.
**摘要**: 通过昆虫细胞表达系统获得高纯度重组KNTC2蛋白，结合X射线晶体学解析其结构，揭示了KNTC2的卷曲螺旋结构域在动粒复合体组装中的关键作用，并提出其突变可能导致染色体不稳定性。
3. **文献名称**: *"Development of a KNTC2-Specific Antibody Using Recombinant Protein as Antigen"*
**作者**: Tanaka H, Nakamura K.
**摘要**: 研究通过原核表达系统(大肠杆菌)表达并纯化KNTC2片段，用于制备多克隆抗体。实验验证了抗体的特异性，并应用于临床样本检测，发现KNTC2在多种癌症中异常高表达。
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**说明**：以上文献为示例性质，实际研究中请通过学术数据库检索具体文献。若需真实文献，建议使用关键词“recombinant KNTC2”“kinetochore KNTC2 expression”在PubMed或Web of Science中查找。
**Background of Recombinant Human KNTC2 Protein**
KNTC2 (Kinetochore-associated protein 2), also known as Nuf2. is a critical component of the Ndc80 complex, which plays an essential role in chromosome segregation during mitosis and meiosis. This protein localizes to the kinetochore, a dynamic structure that connects chromosomes to spindle microtubules, ensuring accurate chromosome alignment and segregation. KNTC2 interacts with other Ndc80 complex members (Ndc80/Hec1. Spc24. and Spc25) to form a conserved four-subunit complex, mediating stable kinetochore-microtubule attachments and regulating the spindle assembly checkpoint (SAC). Dysregulation of KNTC2 is linked to chromosomal instability, aneuploidy, and carcinogenesis, with overexpression observed in multiple cancers, including colorectal, lung, and breast cancers.
Recombinant human KNTC2 protein is engineered for *in vitro* studies to elucidate its structural, functional, and mechanistic roles in cell division. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein retains functional domains, such as coiled-coil regions and microtubule-binding motifs, enabling investigations into kinetochore assembly, SAC signaling, and mitotic error correction. Its applications span biochemical assays (e.g., protein-protein interactions), structural studies, and drug discovery targeting mitotic vulnerabilities in cancer. Research on recombinant KNTC2 enhances understanding of cell cycle regulation and supports the development of therapies for cancers driven by genomic instability.
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