关键信息
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基因名
POU4F1
- 应用
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别名
POU domain. class 4. transcription factor 1. Brain-specific homeobox/POU domain protein 3A. Brain-3A. Brn-3A. Homeobox/POU domain protein RDC-1. Oct-T1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01851
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表达区间
1-419 aa
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氨基酸序列
MMSMNSKQPHFAMHPTLPEHKYPSLHSSSEAIRRACLPTPPLQSNLFASLDETLLARAEALAAVDIAVSQGKSHPFKPDATYHTMNSVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELFNGGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY
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分子量
73.04 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
POU4F1, also known as Brn-3a, is a member of the POU domain family of transcription factors, which play crucial roles in neural development and differentiation. This protein is primarily expressed in the nervous system, particularly in sensory neurons and the retina, where it is involved in the regulation of target gene expression essential for neuronal survival and function. Recent studies have highlighted the pivotal role of POU4F1 in promoting the survival of retinal ganglion cells and its implications in neurodegenerative diseases such as glaucoma. Given its significant involvement in neuronal development and disease processes, researchers have increasingly focused on the production and characterization of recombinant POU4F1 protein. This enables the investigation of its functional properties, interaction with other proteins, and the molecular pathways it regulates. Furthermore, recombinant POU4F1 could serve as a valuable tool for drug discovery and gene therapy approaches aimed at neuroprotection and regeneration, making it a promising candidate for therapeutic interventions in neural disorders. By understanding its structure and function through recombinant technology, researchers aim to elucidate the mechanisms by which POU4F1 contributes to neuronal health and the potential for restoring function in damaged nervous tissue.
