| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KNCN |
| Uniprot No | A6PVL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 活性数据 | MLRQSLSGCPCPALPSCLLPLCQCPPGLGDPDRRQARPCRPQSGWEQCLEWGLLHSPGQASPKWIPLVWAGTWSKVSPDAPTGFPCFRLLPWPFFLDCSSALLPVQSTDLFLWNLGSRALTMTRMPQSLALTRLQK |
| 分子量 | 41.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人KNCN蛋白的模拟参考文献示例(注：KNCN为虚构蛋白名称，文献为模拟生成)：
1. **文献名称**: "Structural characterization of recombinant human KNCN protein and its role in cellular oxidative stress response"
**作者**: Zhang L., et al.
**摘要**: 本研究成功在大肠杆菌中表达重组人KNCN蛋白，并通过晶体学解析其三维结构。功能实验表明KNCN通过调节SOD活性减轻H₂O₂诱导的细胞氧化损伤，提示其在抗氧化治疗中的潜在应用。
2. **文献名称**: "KNCN interacts with PI3K/Akt signaling pathway to promote neuronal survival"
**作者**: Tanaka K., et al.
**摘要**: 通过体外神经元模型证实，重组KNCN蛋白能特异性激活PI3K/Akt通路，显著抑制谷氨酸兴奋毒性引发的细胞凋亡，为神经退行性疾病治疗提供新靶点。
3. **文献名称**: "Recombinant human KNCN ameliorates cardiac fibrosis via TGF-β/Smad pathway inhibition"
**作者**: Wang Y., et al.
**摘要**: 在心肌纤维化小鼠模型中，注射重组KNCN蛋白可降低Col1a1和α-SMA表达，机制研究发现其通过阻断Smad2/3磷酸化抑制TGF-β信号传导，提示其抗纤维化治疗价值。
注：实际研究中若需参考文献，请通过PubMed/Google Scholar检索真实存在的蛋白质名称(如KCN基因家族相关文献)。
**Background of Recombinant Human KNCN Protein**
KNCN (Kinocilin), encoded by the *KNCN* gene in humans, is a cysteine-rich protein implicated in cellular structure and signaling pathways. Predominantly expressed in tissues like the testis, kidney, and brain, KNCN is associated with cilia function and extracellular matrix interactions. Structurally, it contains conserved domains linked to protein-protein interactions, suggesting roles in cellular adhesion, motility, or signal transduction. Aberrant KNCN expression has been observed in certain cancers and ciliopathies, though its precise biological mechanisms remain under investigation.
Recombinant human KNCN protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research. This allows studies on its molecular interactions, structural characterization, and role in diseases. Its recombinant form is critical for developing assays, antibody production, and exploring therapeutic targets, particularly in disorders involving ciliary dysfunction or tissue remodeling.
Current research focuses on elucidating KNCN's involvement in spermatogenesis, renal development, and neuronal processes. As a relatively understudied protein, KNCN represents a potential biomarker or therapeutic candidate, driving interest in its recombinant production to advance both basic and translational studies. Further insights into KNCN could enhance understanding of cilia-related pathologies and tissue-specific regulatory networks.
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