| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLRC4 |
| Uniprot No | O43908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158aa |
| 活性数据 | MNKQRGTYSEVSLAQDPKRQQRKLKGNKISISGTKQEIFQVELNLQNASSDHQGNDKTYHCKGLLPPPEKLTAEVLGIICIVLMATVLKTIVLIPCIGVLEQNNFSLNRRMQKARHCGHCPEEWITYSNSCYYIGKERRTWEERVCWPVLRRTLICFL |
| 分子量 | 43.12 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于重组人KLRC4蛋白的参考文献示例(注：以下内容为虚构演示，实际引用需查阅真实数据库)：
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1. **文献名称**: *Structural and Functional Characterization of Recombinant Human KLRC4 Protein*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组人KLRC4胞外域蛋白，利用X射线晶体学解析了其三维结构，发现其与配体相互作用的关键氨基酸位点，为NK细胞受体功能研究提供结构基础。
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2. **文献名称**: *KLRC4-Mediated Immune Regulation in Viral Infections: Insights from Recombinant Protein Assays*
**作者**: Zhang L, et al.
**摘要**: 研究通过哺乳动物细胞(HEK293)表达重组KLRC4蛋白，体外实验证明其与特定病毒蛋白的相互作用可抑制NK细胞活性，提示KLRC4可能参与病毒逃逸免疫监视的分子机制。
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3. **文献名称**: *Development of a KLRC4-Specific Monoclonal Antibody Using Recombinant Protein Immunization*
**作者**: Tanaka K, et al.
**摘要**: 团队通过CHO细胞表达纯化的重组KLRC4蛋白免疫小鼠，筛选出高特异性单克隆抗体，验证其在流式细胞术和免疫组化中的应用潜力，助力KLRC4的临床检测研究。
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4. **文献名称**: *Proteomic Analysis of KLRC4 Interaction Partners via Affinity Purification*
**作者**: Garcia R, et al.
**摘要**: 采用重组KLRC4蛋白固定化亲和层析技术，鉴定了其在NK细胞裂解物中的潜在结合蛋白，揭示KLRC4可能通过调控SHP-1磷酸酶信号通路影响细胞毒性功能。
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**建议**：实际研究中请通过**PubMed**或**Web of Science**检索真实文献(关键词：recombinant KLRC4、NKG2F protein)。若研究较少，可扩展至KLRC家族(如KLRC1/KLRC2)相关机制或结构同源性分析。
**Background of Recombinant Human KLRC4 Protein**
The killer cell lectin-like receptor subfamily C, member 4 (KLRC4), also known as NKG2F, is a transmembrane protein primarily expressed on natural killer (NK) cells and certain T-cell subsets. It belongs to the NKG2 family of C-type lectin receptors, which play critical roles in modulating immune responses by regulating NK cell activation and cytotoxicity. KLRC4 is encoded within the natural killer gene complex (NKC) on human chromosome 12 and shares structural homology with other NKG2 receptors, featuring a conserved carbohydrate-recognition domain (CRD) that facilitates ligand interaction.
Recombinant human KLRC4 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling studies of its biochemical and functional properties. KLRC4 is known to form heterodimers with CD94. a common adaptor molecule, and this complex may interact with non-classical MHC class I molecules, such as HLA-E, to transmit inhibitory or activating signals. While its precise biological role remains less characterized compared to related receptors (e.g., NKG2A or NKG2D), KLRC4 is hypothesized to contribute to immune surveillance, particularly in viral infections and tumorigenesis, by fine-tuning NK cell responses.
Research on recombinant KLRC4 aids in elucidating its ligand specificity, signaling pathways, and therapeutic potential in immunotherapy. It also serves as a tool for developing antibodies or inhibitors targeting NK cell activity in autoimmune disorders or cancers. Further studies are needed to fully define its physiological and pathological relevance.
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