| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Klkbl4 |
| Uniprot No | Q6PEW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-383aa |
| 活性数据 | MRGVLLVLLGLLYSSTSCGVQKASVFYGPDPKEGLVSSMEFPWVVSLQDSQYTHLAFGCILSEFWVLSIASAIQNRKDIVVIVGISNMDPSKIAHTEYPVNTIIIHEDFDNNSMSNNIALLKTDTAMHFGNLVQSICFLGRMLHTPPVLQNCWVSGWNPTSATGNHMTMSVLRKIFVKDLDMCPLYKLQKTECGSHTKEETKTACLGDPGSPMMCQLQQFDLWVLRGILNFGGETCPGLFLYTKVEDYSKWITSKAERAGPPLSSLHHWEKLISFSHHGPNAAMTQKTYSDSELGHVGSYLQGQRRTITHSRLGNSSRDSLDVREKDVKESGRSPEASVQPLYYDYYGGEVGEGRIFAGQNRLYQPEEIILVSFVLVFFCSSI |
| 分子量 | 69.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设重组人**KLK4**蛋白(可能为用户所指的Klkbl4的笔误)的相关文献示例，供参考：
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1. **文献名称**: *Recombinant human kallikrein 4 expression and characterization in prostate cancer cells*
**作者**: Smith A, et al.
**摘要**: 研究报道了重组人KLK4蛋白在前列腺癌细胞中的稳定表达，证实其通过激活PAR-1受体促进癌细胞侵袭和迁移，提示其作为治疗靶点的潜力。
2. **文献名称**: *Enzymatic activity profiling of recombinant human KLK4 using synthetic substrates*
**作者**: Jones B, et al.
**摘要**: 通过优化表达系统在大肠杆菌中制备重组人KLK4蛋白，分析其蛋白酶活性及底物特异性，发现其对富含精氨酸的肽链具有高度水解活性。
3. **文献名称**: *Role of KLK4 in enamel formation: Insights from recombinant protein studies*
**作者**: Lee C, et al.
**摘要**: 利用重组人KLK4蛋白处理成釉细胞，发现其调控牙釉质基质蛋白水解，为遗传性釉质发育不全的机制研究提供依据。
4. **文献名称**: *Recombinant KLK4 as a potential diagnostic marker in ovarian cancer*
**作者**: Wang D, et al.
**摘要**: 研究开发了高纯度重组人KLK4蛋白，验证其在卵巢癌患者血清中的过表达，并探讨其与肿瘤分期的相关性。
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**注意**：若目标蛋白为非常规命名(如Klkbl4)，可能存在拼写误差或非主流研究领域，建议结合基因数据库(如NCBI)确认名称后进一步检索。上述内容为模拟文献，实际引用需核实具体来源。
Recombinant human KLK4 (Kallikrein-related peptidase 4) is a serine protease encoded by the *KLK4* gene, part of the tissue kallikrein (*KLK*) gene family on chromosome 19. KLK4 is primarily known for its role in physiological processes such as enamel formation, prostate function, and tissue remodeling. It cleaves extracellular matrix proteins and activates other proteases, contributing to processes like tooth enamel maturation during development and seminal clot liquefaction. Dysregulation of KLK4 has been implicated in pathologies including prostate cancer, ovarian cancer, and dental disorders like amelogenesis imperfecta.
Recombinant KLK4 is produced via genetic engineering in systems like mammalian cells or *E. coli*, enabling controlled study of its enzymatic activity, structure, and interactions. Its recombinant form is crucial for research into protease-mediated signaling, cancer metastasis, and as a potential therapeutic or diagnostic target. Recent studies also explore its role in neurodegenerative diseases and immune modulation. However, functional characterization remains ongoing due to challenges in detecting endogenous KLK4 and understanding tissue-specific isoforms. Research tools such as recombinant KLK4 antibodies and activity assays continue to advance its biomedical applications.
(Note: "Klkbl4" is assumed to refer to KLK4. as no widely recognized "Klkbl4" protein exists in current databases. Clarification is recommended.)
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