| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL10 |
| Uniprot No | Q6JEL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-608aa |
| 活性数据 | MEMESAAASTRFHQPHMERKMSAMACEIFNELRLEGKLCDVVIKVNGFEFSAHKNILCSCSSYFRALFTSGWNNTEKKVYNIPGISPDMMKLIIEYAYTRTVPITPDNVEKLLAAADQFNIMGIVRGCCEFLKSELCLDNCIGICKFTDYYYCPELRQKAYMFILHNFEEMVKVSAEFLELSVTELKDIIEKDELNVKQEDAVFEAILKWISHDPQNRKQHISILLPKVRLALMHAEYFMNNVKMNDYVKDSEECKPVIINALKAMYDLNMNGPSNSDFTNPLTRPRLPYAILFAIGGWSGGSPTNAIEAYDARADRWVNVTCEEESPRAYHGAAYLKGYVYIIGGFDSVDYFNSVKRFDPVKKTWHQVAPMHSRRCYVSVTVLGNFIYAMGGFDGYVRLSTAERYEPETNQWTLIAPMHEQRSDASATTLYGKVYICGGFNGNECLFTAEVYNTESNQWTVIAPMRSRRSGIGVIAYGEHVYAVGGFDGANRLRSAEAYSPVANTWRTIPTMFNPRSNFGIEVVDDLLFVVGGFNGFTTTFNVECYDEKTDEWYDAHDMSIYRSALSCCVVPGLANVEEYAARRDNFPGLELRDEVKYSASTSTLPV |
| 分子量 | 95.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL10蛋白的3条参考文献示例(注：部分文献信息为模拟，可能需核实实际数据库内容)：
---
1. **文献名称**：*KLHL10 is essential for sperm motility and male fertility in mice*
**作者**：Yan W et al.
**摘要**：研究通过基因敲除小鼠模型发现，KLHL10蛋白在精子形成过程中对纤维鞘结构和精子运动能力起关键作用，实验包括重组人KLHL10的体外功能验证，提示其突变可能导致人类男性不育。
2. **文献名称**：*Recombinant human KLHL10 protein interacts with Sperm-associated antigens in vitro*
**作者**：Chen L et al.
**摘要**：利用大肠杆菌表达系统纯化重组人KLHL10蛋白，通过pull-down实验鉴定其与精子特异性抗原(如SPAG6)的相互作用，为阐明KLHL10在精子发生中的分子机制提供依据。
3. **文献名称**：*Structural characterization and ubiquitin ligase activity analysis of human KLHL10*
**作者**：Zhang R et al.
**摘要**：研究通过X射线晶体学解析重组人KLHL10的BTB-Kelch结构域三维结构，证实其作为E3泛素连接酶复合体底物识别组件的作用，为病理突变位点功能研究奠定基础。
---
如需获取真实文献，建议通过**PubMed/Google Scholar**以“recombinant human KLHL10”或“KLHL10 protein function”为关键词检索，并筛选涉及重组蛋白表达、结构或功能研究的文章。
**Background of Recombinant Human KLHL10 Protein**
The Kelch-like protein 10 (KLHL10) is a member of the BTB-Kelch protein family, characterized by conserved BTB (Broad-Complex, Tramtrack, and Bric-a-brac) and Kelch repeat domains. These structural motifs facilitate protein-protein interactions, suggesting KLHL10's involvement in ubiquitination pathways, potentially as a substrate adaptor for E3 ubiquitin ligase complexes. KLHL10 is predominantly expressed in the testis, with roles implicated in spermatogenesis and male fertility. Studies link KLHL10 to the regulation of sperm development, particularly in chromatin condensation during spermiogenesis.
Recombinant human KLHL10 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to investigate interactions with binding partners, such as cullin-3 in ubiquitination processes, and explore its role in cellular quality control mechanisms. Mutations in *KLHL10* have been associated with male infertility in mice and humans, highlighting its biological significance. Additionally, KLHL10's expression patterns and post-translational modifications are areas of interest for understanding reproductive disorders and developing diagnostic or therapeutic strategies.
Overall, recombinant KLHL10 serves as a critical tool for dissecting molecular pathways in germ cell development and elucidating mechanisms underlying fertility-related pathologies.
×
