| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC8A |
| Uniprot No | Q8IYD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 活性数据 | MEVPNVKDFQWKRLAPLPSRRVYCSLLETGGQVYAIGGCDDNGVPMDCFEVYSPEADQWTALPRLPTARAGVAVTALGKRIMVIGGVGTNQLPLKVVEMYNIDEGKWKKRSMLREAAMGISVTAKDYRVYAAGGMGLDLRPHNHLQHYDMLKDMWVSLAPMPTPRYAATSFLRGSKIYVLGGRQSKYAVNAFEVFDIETRSWTKFPNIPYKRAFSSFVTLDNHLYSLGGLRQGRLYRQPKFLRTMDVFDMEQGGWLKMERSFFLKKRRADFVAGSLSGRVIVAGGLGNQPTVLETAEAFHPGKNKWEILPAMPTPRCACSSIVVKNCLLAVGGVNQGLSDAVEALCVSDS |
| 分子量 | 65.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHDC8A蛋白的模拟参考文献示例(注：因该蛋白研究较少，部分内容为假设性概括，建议进一步查阅实际数据库验证)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human KLHDC8A in Escherichia coli for Structural Studies"*
**作者**：Chen et al., 2021
**摘要**：本研究描述了使用大肠杆菌系统表达重组人KLHDC8A蛋白的优化方法，并通过亲和层析和尺寸排阻色谱纯化获得高纯度蛋白。研究还分析了其二级结构特征，为后续功能研究奠定基础。
2. **文献名称**：*"KLHDC8A Interacts with Cullin-RING Ubiquitin Ligases: Implications for Tumor Suppression"*
**作者**：Tanaka et al., 2019
**摘要**：通过重组KLHDC8A蛋白的体外结合实验，发现其作为底物识别组件与Cullin家族泛素连接酶结合，可能参与细胞周期调控。研究提示KLHDC8A在结直肠癌中表达异常，或具有肿瘤抑制功能。
3. **文献名称**：*"Crystal Structure Analysis of KLHDC8A Reveals a Novel β-Propeller Fold"*
**作者**：Gupta et al., 2020
**摘要**：利用重组KLHDC8A蛋白解析了其晶体结构，发现其Kelch结构域形成典型的β-螺旋桨构象，并预测了可能的配体结合位点，为靶向药物设计提供结构依据。
4. **文献名称**：*"KLHDC8A as a Potential Biomarker for Neurodegenerative Diseases: Insights from Recombinant Protein-Based Assays"*
**作者**：Li et al., 2022
**摘要**：研究通过重组KLHDC8A蛋白开发了ELISA检测方法，发现其在阿尔茨海默病患者脑脊液中表达水平显著升高，提示其可能参与神经退行性病变的蛋白稳态调控。
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**注意**：上述文献为示例性质，具体内容需以实际检索结果为准。建议通过PubMed、Web of Science等平台以关键词“recombinant KLHDC8A”或“KLHDC8A protein function”进行查询。
**Background of Recombinant Human KLHDC8A Protein**
KLHDC8A (Kelch Domain-Containing Protein 8A) is a member of the Kelch superfamily, characterized by conserved Kelch repeat domains that typically form β-propeller structures to mediate protein-protein interactions. This protein is implicated in diverse cellular processes, including ubiquitination pathways, where it may act as a substrate recognition component of E3 ubiquitin ligase complexes, targeting specific proteins for proteasomal degradation. While its precise physiological roles remain under investigation, KLHDC8A has been linked to transcriptional regulation, cell cycle control, and stress response mechanisms. Dysregulation of KLHDC8A expression has been observed in certain cancers and neurological disorders, suggesting potential involvement in disease pathogenesis.
Recombinant human KLHDC8A protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional and structural studies. Its purified form facilitates investigations into binding partners, enzymatic activity, and post-translational modifications. Researchers also utilize it to explore its role in ubiquitin-mediated signaling, disease models, and therapeutic target validation. Ongoing research aims to unravel its mechanistic contributions to cellular homeostasis and pathology, with potential applications in biomarker discovery or targeted therapy development.
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