| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC7B |
| Uniprot No | Q96G42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495aa |
| 活性数据 | MPRGPAQPPAQRPPGPAASSSARRSQPVPQLRKRSRCEIAPSSEQEVRPAASGDPQGEAPGEGGSPAGRSGALTEKQEEARKLMVFLQRPGGWGVVEGPRKPSSRALEPATAAALRRRLDLGSCLDVLAFAQQHGEPGLAQETYALMSDNLLRVLGDPCLYRRLSAADRERILSLRTGRGRAVLGVLVLPSLYQGGRSGLPRGPRGEEPPAAAPVSLPLPAHLHVFNPRENTWRPLTQVPEEAPLRGCGLCTMHNYLFLAGGIRGSGAKAVCSNEVFCYNPLTNIWSQVRPMQQARAQLKLVALDGLLYAIGGECLYSMECYDPRTDAWTPRAPLPAGTFPVAHEAVACRGDIYVTGGHLFYRLLRYSPVKDAWDECPYSASHRRSSDIVALGGFLYRFDLLRGVGAAVMRYNTVTGSWSRAASLPLPAPAPLHCTTLGNTIYCLNPQVTATFTVSGGTAQFQAKELQPFPLGSTGVLSPFILTLPPEDRLQTSL |
| 分子量 | 79.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHDC7B蛋白的3篇参考文献示例(注：内容为虚构，实际文献需通过学术数据库查询)：
---
**1. 文献名称**：*Structural characterization and functional analysis of recombinant human KLHDC7B protein*
**作者**：Zhang Y, Chen L, Wang X
**摘要**：本研究成功表达并纯化了重组人KLHDC7B蛋白，通过X射线晶体学解析其Kelch结构域的三维结构。实验表明，KLHDC7B可能通过识别底物蛋白中的特定基序参与泛素化途径的调控，为癌症治疗的靶点筛选提供了基础。
---
**2. 文献名称**：*KLHDC7B modulates oxidative stress response via interaction with Nrf2: Insights from recombinant protein assays*
**作者**：Tanaka K, Suzuki M, Nakamura S
**摘要**：利用重组人KLHDC7B蛋白进行体外结合实验，发现其直接与转录因子Nrf2相互作用，调控抗氧化反应元件(ARE)的活性。该研究揭示了KLHDC7B在氧化应激和神经退行性疾病中的潜在作用。
---
**3. 文献名称**：*Recombinant KLHDC7B as a biomarker for early-stage colorectal cancer: A proteomic approach*
**作者**：Garcia R, Müller P, Lee JH
**摘要**：通过高通量蛋白质组学分析，发现重组KLHDC7B在结直肠癌患者血清中异常表达。研究表明，KLHDC7B可能通过Wnt/β-catenin通路促进肿瘤增殖，其重组蛋白可作为一种新型诊断标志物。
---
**4. 文献名称**：*High-yield expression and purification of recombinant KLHDC7B in E. coli for biochemical studies*
**作者**：Liu Q, Zhang H, Zhou W
**摘要**：优化重组人KLHDC7B在大肠杆菌中的表达系统，实现了高效可溶性表达。纯化蛋白成功用于酶动力学分析，揭示其底物结合特性及在代谢调控中的潜在功能。
---
如需真实文献，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索关键词“recombinant KLHDC7B”或结合具体研究领域筛选。
**Recombinant human KLHDC7B protein** is a genetically engineered form of the Kelch domain-containing protein 7B, encoded by the *KLHDC7B* gene. The KLHDC protein family is characterized by the presence of Kelch repeat domains, which typically form β-propeller structures involved in protein-protein interactions, substrate recognition, or scaffolding roles in cellular processes. While the precise biological function of KLHDC7B remains incompletely characterized, studies suggest its potential involvement in ubiquitination pathways, cancer progression, or neurodevelopmental regulation. Some evidence links KLHDC7B overexpression to hepatocellular carcinoma or glioblastoma, though mechanistic details are still under investigation.
Recombinant KLHDC7B is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells like HEK293) to enable large-scale purification for functional and structural studies. This allows researchers to explore its interactome, enzymatic properties, or regulatory roles in vitro. The recombinant protein is often tagged with affinity markers (e.g., His-tag, GST) to facilitate purification and detection. Current research focuses on clarifying its molecular targets, post-translational modifications, and relevance to diseases, with potential applications in biomarker discovery, drug development, or elucidating pathways in cellular stress responses. Its study contributes to understanding the expanding roles of Kelch domain proteins in human health and disease.
×
