| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC6 |
| Uniprot No | Q3ZCT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 活性数据 | MECKIEGKEK YQHSLNLLNK IQNMKELAEM IDVVLTAEGE KFPCHRLVLA AFSPYFKAMF TCGLLECNQR EVILYDITAE SVSVLLNYMY NAALEINNAN VQTVAMAAYF MQMEEVFSVC QKYMMDHMDA SNCLGIYYFA KQIGAEDLSD RSKKYLYQHF AEVSLHEEIL EIEVHQFLTL IKSDDLNISR EESILDLVLR WVNHNKELRT VHLVELLKQV RLELVNPSFL RQALRRNTML LCDADCVDII QNAFKAIKTP QQHSLNLRYG METTSLLLCI GNNSSGIRSR HRSYGDASFC YDPVSRKTYF ISSPKYGEGL GTVCTGVVME NNTIIVAGEA SASKLSRQKN KNVEIYRYHD RGNQFWEKLC TAEFRELYAL GSIHNDLYVI GGQMKIKNQY LITNCVDKYS VERDNWKRVS PLPLQLACHA VVTVNNKLYV IGGWTPQMDL PDEEPDRLSN KLLQYDPSQD QWSVRAPMKY SKYRFSTAVV NSEIYVLGGI GCVGQDKGQV RKCLDVVEIY NPDGDFWREG PPMPSPLLSL RTNSTNAGAV DGKLYVCGGF HGADRHEVIS KEILELDPWE NQWNVVAINV LMHDSYDVCL VARMNPRDLI PPPSDLVEEG NEH |
| 分子量 | 71 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHDC6蛋白的3篇代表性文献的简要信息(文献标题和作者为虚构示例，仅供格式参考)：
1. **文献名称**：*Structural and functional analysis of recombinant human KLHDC6 in ubiquitin ligase complexes*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人KLHDC6蛋白，解析了其Kelch结构域的三维结构，发现其通过Cullin3形成E3泛素连接酶复合体，并参与特异性底物的泛素化降解。
2. **文献名称**：*KLHDC6 interacts with mitochondrial proteins via its kelch domain*
**作者**：Li X, Wang Y.
**摘要**：利用哺乳动物细胞表达的重组KLHDC6蛋白，结合质谱分析发现其与线粒体相关蛋白相互作用，提示KLHDC6可能通过调控线粒体功能参与细胞凋亡通路。
3. **文献名称**：*Expression and purification of recombinant KLHDC6 for high-throughput drug screening*
**作者**：Chen R, et al.
**摘要**：优化了KLHDC6蛋白在昆虫杆状病毒系统中的重组表达和纯化工艺，开发了基于该蛋白的体外泛素化活性检测平台，用于筛选潜在的小分子抑制剂。
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**备注**：实际文献需通过PubMed或Google Scholar检索关键词(如“KLHDC6 recombinant”“KLHDC6 ubiquitin ligase”)。若研究较少，可扩展至KLHDC家族其他成员(如KLHDC2/3)的文献作为参考。
Recombinant human KLHDC6 protein is a genetically engineered form of the Kelch domain-containing protein 6. encoded by the KLHDC6 gene. Belonging to the Kelch superfamily, KLHDC6 features multiple Kelch motifs that form β-propeller structures, which mediate protein-protein interactions and substrate recognition. The protein is implicated in cellular processes such as ubiquitination, where it potentially acts as a substrate adaptor in E3 ubiquitin ligase complexes, targeting specific proteins for proteasomal degradation. Though its biological functions remain understudied, KLHDC6 is hypothesized to play roles in cell cycle regulation, oxidative stress response, and neuronal development based on structural homology with related Kelch proteins.
Recombinant KLHDC6 is typically produced in bacterial or mammalian expression systems, enabling controlled study of its biochemical properties and interactions. Its applications span structural studies, interaction mapping (e.g., with Cullin-RING ligases), and functional assays to elucidate its role in disease pathways. Emerging evidence links KLHDC6 dysregulation to cancer progression and neurodevelopmental disorders, driving interest in its therapeutic potential. The recombinant form overcomes challenges of low endogenous protein abundance, providing a standardized tool for mechanistic research and drug discovery pipelines.
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