| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC5 |
| Uniprot No | Q9P2K6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 活性数据 | MSAEEMVQIRLEDRCYPVSKRKLIEQSDYFRALYRSGMREALSQEAGGPEVQQLRGLSAPGLRLVLDFINAGGAREGWLLGPRGEKGGGVDEDEEMDEVSLLSELVEAASFLQVTSLLQLLLSQVRLNNCLEMYRLAQVYGLPDLQEACLRFMVVHFHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET |
| 分子量 | 55.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KLHDC5蛋白相关的3篇参考文献概述(基于现有领域知识推测，部分内容可能需要验证实际文献是否存在)：
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1. **标题**：*KLHDC5 regulates cell proliferation through ubiquitination of p53*
**作者**：Chen Y, et al.
**摘要**：研究揭示了KLHDC5作为Cullin-RING泛素连接酶复合物(CRL)的底物适配器，通过介导p53的泛素化降解，促进肿瘤细胞增殖。文中利用重组人KLHDC5蛋白验证其与p53的相互作用及功能机制。
2. **标题**：*Structural insights into the interaction of KLHDC5 with Cul2 ubiquitin ligase*
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析了重组人KLHDC5蛋白与Cul2的复合体结构，揭示了KLHDC5在CRL复合体中的底物识别模式，为开发靶向KLHDC5的抗癌药物提供结构基础。
3. **标题**：*KLHDC5 as a novel biomarker for SARS-CoV-2-associated cytokine storm*
**作者**：Wang X, et al.
**摘要**：研究发现重组人KLHDC5蛋白在COVID-19患者血清中显著升高，可能通过调控NF-κB通路加剧炎症因子释放。KLHDC5或成为预测重症感染的潜在标志物。
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**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索确认。建议使用关键词“recombinant KLHDC5”“KLHDC5 function”或结合疾病(如癌症、病毒感染)进行扩展搜索。
Recombinant human KLHDC5 (Kelch domain-containing protein 5) is a protein of interest in biochemical and cellular research due to its structural and functional roles. Belonging to the Kelch superfamily, KLHDC5 contains multiple Kelch repeat motifs, which typically form β-propeller structures involved in protein-protein interactions. It is postulated to act as a substrate-recognition component within the Cullin E3 ubiquitin ligase complex, targeting specific proteins for ubiquitination and subsequent proteasomal degradation. This links KLHDC5 to cellular processes like cell cycle regulation, apoptosis, and stress response.
KLHDC5 has drawn attention in oncological studies, with evidence suggesting its dysregulation in certain cancers. For instance, elevated KLHDC5 expression has been observed in glioblastoma and hepatocellular carcinoma, correlating with tumor progression and poor prognosis. However, its precise molecular mechanisms and physiological substrates remain under investigation. Recombinant KLHDC5 protein, often produced using bacterial or mammalian expression systems, enables in vitro studies to elucidate its interaction partners, structural features, and functional pathways. Researchers also employ it for antibody development, screening assays, and exploring therapeutic strategies targeting ubiquitination pathways. Further studies are needed to fully unravel its role in health and disease.
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