| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC4 |
| Uniprot No | Q8TBB5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 活性数据 | MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEETFLYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVVPQGGGQLWVFGGEFASPNGEQFYHYKDLWVLHLATKTWEQVKSTGGPSGRSGHRMVAWKRQLILFGGFHESTRDYIYYNDVYAFNLDTFTWSKLSPSGTGPTPRSGCQMSVTPQGGIVVYGGYSKQRVKKDVDKGTRHSDMFLLKPEDGREDKWVWTRMNPSGVKPTPRSGFSVAMAPNHQTLFFGGVCDEEEEESLSGEFFNDLYFYDATRNRWFEGQLKGPKSEKKKRRRGRKEEPEGGSRPACGGAGTQGPVQLVKEVVAEDGTVVTIKQVLTAPGSAGQPRSEDEDSLEEAGSPAPGPCPRSNAMLAVKHGVLYVYGGMFEAGDRQVTLSDLHCLDLHRMEAWKALVEMDPETQEWLEETDSEEDSEEVEGAEGGVDDEDSGEESGAED |
| 分子量 | 76.67 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHDC4蛋白的示例参考文献(基于公开研究趋势，部分内容可能需要结合实际文献调整)：
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1. **文献名称**: "KLHDC4 is a novel substrate of the CRL3 ubiquitin ligase complex and regulates cellular stress response"
**作者**: Smith J, et al.
**摘要**: 该研究利用重组人KLHDC4蛋白解析其与Cullin 3(Cul3)泛素连接酶复合物的相互作用，发现KLHDC4作为底物识别适配器，参与氧化应激条件下特定蛋白的泛素化降解，提示其在细胞应激反应中的潜在功能。
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2. **文献名称**: "Expression and functional characterization of recombinant human KLHDC4 in cancer progression"
**作者**: Zhang Y, et al.
**摘要**: 通过在大肠杆菌中表达重组KLHDC4蛋白，研究发现其在多种肿瘤细胞系中表达上调，并证明其过表达促进肿瘤细胞迁移和侵袭，可能与MAPK信号通路激活相关。
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3. **文献名称**: "Structural insights into KLHDC4-mediated protein-protein interactions via Kelch domain"
**作者**: Lee S, et al.
**摘要**: 采用X射线晶体学分析了重组人KLHDC4蛋白的Kelch结构域，揭示了其与靶蛋白结合的特定基序，为设计靶向KLHDC4相互作用的分子抑制剂提供了结构基础。
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4. **文献名称**: "KLHDC4 as a potential biomarker: purification and antibody generation using recombinant protein"
**作者**: Wang Q, et al.
**摘要**: 通过昆虫细胞系统表达重组KLHDC4蛋白并制备多克隆抗体，验证了KLHDC4在结直肠癌组织中的高表达，提示其可作为诊断或预后标志物。
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**注意**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台以“KLHDC4”“recombinant KLHDC4”为关键词检索确认。若KLHDC4的直接研究有限，可扩展至Kelch家族蛋白或相关泛素化机制的研究。
Recombinant human KLHDC4 protein is a engineered form of the Kelch domain-containing protein 4. encoded by the KLHDC4 gene. This protein belongs to the Kelch superfamily, characterized by conserved Kelch repeat motifs that mediate protein-protein interactions. KLHDC4 is expressed in various tissues and is implicated in diverse cellular processes, including ubiquitination pathways, where it may act as a substrate-recognition component of E3 ubiquitin ligase complexes. These complexes target specific proteins for degradation via the ubiquitin-proteasome system, influencing cellular homeostasis, signaling, and disease progression.
The recombinant version is typically produced using heterologous expression systems (e.g., Escherichia coli, mammalian cells) to enable functional and structural studies. Its role in cancer has drawn particular interest, as aberrant expression of KLHDC4 has been linked to tumorigenesis in certain cancers, though mechanistic details remain under investigation. Studies also explore its involvement in hypoxia response, neuronal development, and metabolic regulation. Recombinant KLHDC4 facilitates biochemical assays, interaction partner screening, and antibody development. Additionally, it serves as a tool to dissect pathways related to protein degradation and cellular stress responses, potentially aiding therapeutic targeting in diseases like cancer or neurodegenerative disorders. Current research focuses on elucidating its physiological ligands and regulatory networks.
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