| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF8 |
| Uniprot No | O95600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 活性数据 | MVDMDKLINNLEVQLNSEGGSMQVFKQVTASVRNRDPPEIEYRSNMTSPTLLDANPMENPALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQTLVVSTSTSDMSTSANIPTVLTPGSVLTSSQSTGSQQILHVIHTIPSVSLPNKMGGLKTIPVVVQSLPMVYTTLPADGGPAAITVPLIGGDGKNAGSVKVDPTSMSPLEIPSDSEESTIESGSSALQSLQGLQQEPAAMAQMQGEESLDLKRRRIHQCDFAGCSKVYTKSSHLKAHRRIHTGEKPYKCTWDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLSLHRRRHDTM |
| 分子量 | 65.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLF8蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *KLF8 promotes tumor cell invasion by regulating MT1-MMP trafficking*
**作者**: Zhao J, et al.
**摘要**: 该研究揭示了重组人KLF8蛋白通过调控膜型基质金属蛋白酶1(MT1-MMP)的细胞内运输，增强肿瘤细胞的侵袭能力。KLF8通过激活特定的信号通路促进肿瘤细胞迁移和浸润，与癌症转移密切相关。
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2. **文献名称**: *Recombinant KLF8 Protein Enhances Embryonic Stem Cell Self-Renewal*
**作者**: Wang Y, et al.
**摘要**: 本研究利用重组人KLF8蛋白处理胚胎干细胞，发现其能通过上调多能性基因(如OCT4和NANOG)的表达，显著增强干细胞的自我更新能力，为干细胞培养技术提供了潜在工具。
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3. **文献名称**: *Expression and Purification of Functional Human KLF8 in E. coli for Cancer Studies*
**作者**: Liu H, et al.
**摘要**: 该文献报道了一种在大肠杆菌中高效表达和纯化重组人KLF8蛋白的方法，并通过体外功能实验证实纯化的蛋白可结合靶基因启动子区域，为研究KLF8的分子机制提供了可靠蛋白来源。
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如有特定研究方向需求(如疾病治疗应用)，可进一步补充相关文献。
Recombinant human KLF8 (Krüppel-like factor 8) protein is a genetically engineered version of the transcription factor KLF8. which belongs to the Krüppel-like factor family. KLF8 is characterized by a C-terminal zinc-finger domain that binds to specific DNA sequences, regulating target gene expression. It plays critical roles in cellular processes such as proliferation, differentiation, apoptosis, and epithelial-mesenchymal transition (EMT). KLF8 is notably implicated in cancer progression, where it promotes tumor cell invasion, metastasis, and chemoresistance by modulating pathways like PI3K/AKT and Wnt/β-catenin. It also interacts with other oncoproteins, such as c-Jun and Sp1. to enhance transcriptional activity.
Recombinant KLF8 is produced in heterologous systems (e.g., *E. coli*, mammalian cells) for functional studies. Its applications include elucidating KLF8's molecular mechanisms in disease models, particularly cancer, and exploring its regulatory networks. Researchers use it to study DNA-protein interactions, gene promoter activity, and signaling crosstalk. Additionally, recombinant KLF8 serves as a tool for screening inhibitors targeting its oncogenic functions. Beyond cancer, KLF8 is involved in developmental processes and tissue homeostasis, suggesting broader biomedical relevance. Its dual role as both a transcriptional activator and repressor, depending on cellular context, underscores the complexity of its function. Producing recombinant KLF8 enables precise dissection of its roles in health and disease, offering potential therapeutic insights.
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