| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLC4 |
| Uniprot No | Q9NSK0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 活性数据 | MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVLQSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASCYLKQGKYAEAETLYKEILTRAHVQEFGSVDDDHKPIWMHAEEREEMSKSRHHEGGTPYAEYGGWYKACKVSSPTVNTTLRNLGALYRRQGKLEAAETLEECALRSRRQGTDPISQTKVAELLGESDGRRTSQEGPGDSVKFEGGEDASVAVEWSGDGSGTLQRSGSLGKIRDVLRRSSELLVRKLQGTEPRPSSSNMKRAASLNYLNQPSAAPLQVSRGLSASTMDLSSSS |
| 分子量 | 95 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLC4蛋白的参考文献示例(文献信息为模拟，供参考)：
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1. **文献名称**：*Cloning and Functional Characterization of Human KLC4: Implications for Cargo Binding in Intracellular Transport*
**作者**：Smith A, et al.
**摘要**：本研究克隆并表达了重组人KLC4蛋白，揭示了其与驱动蛋白重链(Kinesin-1)的相互作用，并证明KLC4通过TPR结构域特异性识别膜结合细胞器上的信号基序，参与轴突运输调控。
2. **文献名称**：*Structural Analysis of Recombinant KLC4 Reveals a Dimeric Assembly Required for Motor Complex Stability*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了重组KLC4蛋白的晶体结构，发现其形成二聚体并与微管结合，阐明了KLC4二聚化对驱动蛋白复合物稳定性及微管导向运输的重要性。
3. **文献名称**：*Recombinant KLC4 Modulates Amyloid Precursor Protein Trafficking in Alzheimer's Disease Models*
**作者**：Wang Y, et al.
**摘要**：利用重组KLC4蛋白研究其与淀粉样前体蛋白(APP)的相互作用，发现KLC4表达异常导致APP异常运输，可能通过调节高尔基体-细胞膜转运影响阿尔茨海默病病理进程。
4. **文献名称**：*Development of a High-Yield Expression System for Recombinant Human KLC4 in E. coli*
**作者**：Zhang R, et al.
**摘要**：优化了大肠杆菌中重组KLC4的可溶性表达和纯化流程，验证了重组蛋白的生物学活性，为后续功能研究提供可靠方法学基础。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以“recombinant human KLC4”等关键词检索最新文献，并结合研究领域筛选相关论文。
Recombinant human KLC4 protein is a engineered form of the kinesin light chain 4 (KLC4), a member of the kinesin motor protein family. Kinesins are microtubule-dependent molecular motors responsible for intracellular transport, organelle positioning, and cell division. The KLC4 protein, encoded by the KLC4 gene, functions as a regulatory subunit that associates with kinesin heavy chains (KHCs) to mediate cargo binding and modulate motor activity. Structurally, KLC4 contains tetratricopeptide repeat (TPR) domains that facilitate interactions with specific cargo receptors or adaptor proteins, enabling selective transport of vesicles, organelles, and RNA-protein complexes along microtubules.
KLC4 is expressed in various tissues, with potential roles in neuronal function, secretion, and cell signaling. Dysregulation of kinesin-mediated transport has been implicated in neurodegenerative diseases, cancer metastasis, and developmental disorders. Recombinant KLC4 is typically produced in bacterial or eukaryotic expression systems to study its biochemical properties, cargo-binding specificity, and interactions with KHCs. Its applications include in vitro transport assays, structural analysis (e.g., crystallography or cryo-EM), and drug discovery targeting intracellular trafficking pathways. By enabling controlled experimental access to KLC4. recombinant protein technology enhances our understanding of microtubule-based transport mechanisms and their pathophysiological implications.
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