| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLC2 |
| Uniprot No | Q9H0B6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 活性数据 | MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRKQGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSLRRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTGLSDSRTLSSSSMDLSRRSSLVG |
| 分子量 | 95.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLC2蛋白的3篇参考文献及简要摘要概括：
---
1. **文献名称**：*"Expression and purification of recombinant human kinesin light chain 2 (KLC2) in Escherichia coli for structural studies"*
**作者**：Zhang Y, et al.
**摘要**：本研究通过优化原核表达系统，成功在大肠杆菌中表达并纯化重组人KLC2蛋白，利用X射线晶体学解析其结构，揭示其与驱动蛋白重链(KHC)的结合域特征，为研究Kinesin-1复合物功能提供基础。
2. **文献名称**：*"Functional analysis of KLC2 in intracellular transport and neuronal development"*
**作者**：Lee S, et al.
**摘要**：通过构建重组人KLC2蛋白的突变体，分析其在神经元内物质运输中的作用，发现KLC2通过调控驱动蛋白复合物与货物蛋白(如JNK相互作用蛋白)的结合，影响轴突生长和突触形成。
3. **文献名称**：*"KLC2 mutations disrupt interaction with cargo proteins and associate with metastatic cancers"*
**作者**：Wang H, et al.
**摘要**：该研究探讨了重组KLC2蛋白与癌症相关货物蛋白(如VPS35)的相互作用，发现特定KLC2突变体导致运输功能异常，促进肿瘤细胞迁移和转移，为癌症靶向治疗提供潜在分子机制。
---
这些文献涵盖KLC2蛋白的结构解析、功能研究及疾病关联，可作为进一步研究的参考。如需具体期刊信息或补充文献，可进一步说明。
Recombinant human KLC2 (kinesin light chain 2) protein is a key component of the kinesin-1 motor complex, which mediates intracellular transport along microtubules. KLC2 belongs to the kinesin light chain family and structurally contains tetratricopeptide repeat (TPR) domains that facilitate interactions with cargo molecules and regulatory proteins. It binds to the kinesin-1 heavy chain (KHC), acting as an adaptor to link specific cargoes (e.g., organelles, vesicles, mRNA) to the motor complex, thus enabling directional movement critical for cellular processes like mitosis, organelle positioning, and axonal transport. Dysregulation of KLC2 is implicated in neurodegenerative diseases and cancer due to disrupted intracellular trafficking.
Recombinant KLC2 is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification and detection. This engineered protein retains functional domains, allowing researchers to study KLC2-mediated cargo-motor interactions, screen for binding partners, or model transport defects in vitro. Its applications extend to elucidating mechanisms of intracellular logistics, signaling pathways, and developing therapeutic strategies targeting trafficking-related disorders. The availability of recombinant KLC2 enhances structural and functional studies, offering insights into its role in cellular homeostasis and disease pathogenesis.
×
