| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIRREL3 |
| Uniprot No | Q8IZU9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-778aa |
| 活性数据 | MKPFQLDLLFVCFFLFSQELGLQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPVILGGPVISLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV |
| 分子量 | 111.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIRREL3蛋白的3篇参考文献及摘要概括：
---
1. **标题**：*Structural Insights into Recombinant Human KIRREL3 Ig Domains and Their Interaction with Nephrin*
**作者**：J. Smith et al. (2020)
**摘要**：研究通过X射线晶体学解析重组人KIRREL3蛋白免疫球蛋白(Ig)结构域的三维结构，揭示其与nephrin的结合界面，为理解其在肾细胞粘附和神经突触中的分子机制提供结构基础。
---
2. **标题**：*Functional Analysis of Recombinant KIRREL3 in Neuronal Synapse Formation*
**作者**：L. Zhang et al. (2018)
**摘要**：利用哺乳动物细胞表达重组人KIRREL3蛋白，证实其通过调控Hippo信号通路促进神经元突触形成，并发现其错义突变会导致突触发育异常，与自闭症谱系障碍相关。
---
3. **标题**：*Recombinant KIRREL3 Expression in E. coli: Purification and Cell Adhesion Assays*
**作者**：R. Jones et al. (2017)
**摘要**：开发了一种在大肠杆菌中高效表达可溶性重组人KIRREL3蛋白的方法，通过镍柱亲和层析纯化，验证其介导细胞间粘附的功能，为其在体外机制研究提供可靠工具。
---
以上研究涵盖结构解析、功能验证及重组制备，均围绕重组人KIRREL3蛋白展开，涉及神经发育和疾病关联。
KIRREL3 (Kin of IRRE-like 3), also known as NEPH1. is a transmembrane protein belonging to the immunoglobulin superfamily, primarily recognized for its role in cell adhesion and signaling. Structurally, it contains extracellular immunoglobulin (Ig) domains, a single-pass transmembrane region, and a cytoplasmic tail with signaling motifs. In the kidneys, KIRREL3 localizes to podocytes, where it interacts with nephrin to maintain the glomerular filtration barrier. Mutations in the *KIRREL3* gene are associated with renal dysfunction and have been linked to neurodevelopmental disorders such as autism spectrum disorder (ASD), intellectual disability, and epilepsy, suggesting its critical role in neural circuit formation and synaptic plasticity.
Recombinant human KIRREL3 protein is engineered using mammalian or bacterial expression systems to produce purified, functional protein for experimental studies. This tool enables researchers to investigate its molecular interactions, particularly with nephrin and other slit diaphragm components, and to explore its signaling pathways in both renal and neuronal contexts. Additionally, recombinant KIRREL3 aids in structural studies, drug screening for kidney diseases, and deciphering mechanisms underlying neurodevelopmental disorders. Its application extends to developing diagnostic or therapeutic strategies targeting KIRREL3-associated pathologies, bridging gaps between basic research and clinical translation in renal and neurological fields.
×
