| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIRREL2 |
| Uniprot No | Q6UWL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 活性数据 | MLRMRVPALLVLLFCFRGRAGPSPHLLQQPEDLVVLLGEGGAQASLGRRASASFSEQKNLMRIPGSSDGSSSRGPEEEETGSREDRGPIVHTDHSDLVLEEEGTLETKDPTNGYYKVRGVSVSLSLGEAPGGGLFLPPPSPLGPPGTPTFYDFNPHLGMVPPCRLYRARAGYLTTPHPRAFTSYIKPTSFGPPDLAPGTPPFPYAAFPTPSHPRLQTHV |
| 分子量 | 49.83 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIRREL2蛋白的参考文献示例(注：以下内容为模拟生成，仅供参考)：
1. **文献名称**：*Expression and purification of recombinant human KIRREL2 in HEK293 cells and its interaction with nephrin*
**作者**：Zhang Y, Li H, Wang L.
**摘要**：本研究成功在HEK293细胞中表达并纯化了重组人KIRREL2蛋白，验证其与nephrin的相互作用，揭示其在肾小球足细胞黏附中的潜在功能。
2. **文献名称**：*Structural analysis of the KIRREL2 extracellular domain reveals novel ligand-binding motifs*
**作者**：Smith JR, Tanaka K, Wei S.
**摘要**：通过X射线晶体学解析KIRREL2胞外区结构，发现其独特的Ig样结构域及潜在配体结合位点，为研究其在发育和癌症中的信号传导提供依据。
3. **文献名称**：*KIRREL2 promotes epithelial-mesenchymal transition in colorectal cancer via EGFR signaling*
**作者**：Chen X, Liu Q, Zhou M.
**摘要**：通过重组KIRREL2蛋白功能实验，证明其与EGFR结合并激活下游MAPK通路，促进肿瘤细胞迁移和转移。
4. **文献名称**：*Functional characterization of the cytoplasmic domain of KIRREL2 in podocyte foot processes*
**作者**：Gupta R, Müller D, Johnson A.
**摘要**：研究重组KIRREL2蛋白的胞内结构域功能，发现其通过调控actin细胞骨架重组影响足细胞形态和肾脏滤过屏障完整性。
**提示**：以上文献为模拟生成，建议通过PubMed、Web of Science或Google Scholar检索真实文献，使用关键词“recombinant KIRREL2”“KIRREL2 function”等。
KIRREL2 (Kin of IRRE-like 2), also known as FILT (Filtrin), is a transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules. It shares structural homology with nephrin, a slit diaphragm protein critical for kidney glomerular filtration. Human KIRREL2 is encoded by the *KIRREL2* gene located on chromosome 19q13.12 and is primarily expressed in podocytes, specialized epithelial cells of the kidney, as well as in the nervous system and testes.
Functionally, KIRREL2 mediates cell-cell adhesion through homophilic or heterophilic interactions, playing roles in tissue morphogenesis and cellular signaling. Its extracellular domain contains immunoglobulin-like motifs that facilitate binding, while the intracellular domain interacts with cytoskeletal components and signaling molecules. In the kidney, KIRREL2 localizes to the slit diaphragm, suggesting a role in maintaining filtration barrier integrity, though its mechanisms remain less characterized than nephrin.
Recombinant human KIRREL2 protein is produced using expression systems (e.g., mammalian cells, *E. coli*) for in vitro studies. It enables exploration of its binding partners, structural features, and involvement in diseases like proteinuric kidney disorders or cancers. Research also investigates its potential as a biomarker or therapeutic target. By providing a controlled source of the protein, recombinant KIRREL2 supports functional assays, antibody development, and mechanistic studies of cell adhesion pathways.
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